Production of discrete high specific activity DNA probes using the polymerase chain reaction

Abstract

Conditions are described for the synthesis of discrete DNA probes with high specific activity using the polymerase chain reaction. This method enables the production of DNA probes between any two oligonucleotide sequences from cloned or uncloned templates. These probes are uniform in length and their specific activity (1 × 10⁹ cpm/μg) is comparable to probes produced by other methods.