Role of excitatory amino acid receptors in synaptic transmission in area CA1 of rat hippocampus.

S N Davies, G L Collingridge
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引用次数: 117

Abstract

The new antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), which blocks responses to kainate and quisqualate, has been used in conjunction with D-2-amino-5-phosphonovalerate (APV), which blocks selectively responses to N-methyl-D-aspartate (NMDA), to determine the role of excitatory amino acid receptors in synaptic transmission. An excitatory postsynaptic potential (EPSP)-inhibitory postsynaptic potential (IPSP) sequence was evoked in CA1 neurons by stimulation of the Schaffer collateral-commissural pathway in rat hippocampal slices. CNQX (10 microM) substantially reduced the EPSP without having any effect on input resistance or membrane potential. The IPSP was also reduced provided that the stimulating electrode was place approximately 1 mm from the recording electrode. The EPSP that remained in the presence of CNQX had characteristics of an NMDA receptor-mediated potential; it had a slow timecourse, summated at high frequencies, was blocked reversibly by APV, increased greatly in size in Mg2+-free medium, and showed an anomalous voltage dependence in Mg2+-containing medium. In the presence of CNQX, an APV-sensitive polysynaptic GABAergic IPSP could be evoked, indicating that NMDA receptors can mediate suprathreshold EPSPS in inhibitory interneurons. It is suggested that either NMDA or non-NMDA receptors can, under different circumstances, mediate the synaptic excitation of pyramidal neurons and inhibitory interneurons in area CA1 of the hippocampus.

兴奋性氨基酸受体在大鼠海马CA1区突触传递中的作用。
新的拮抗剂6-氰-7-硝基喹啉-2,3-二酮(CNQX)可以阻断对海碱盐和准qualate的反应,与d -2-氨基-5-磷酸戊酸(APV)联合使用,可以阻断对n -甲基-d -天冬氨酸(NMDA)的选择性反应,以确定兴奋性氨基酸受体在突触传递中的作用。刺激大鼠海马Schaffer侧交通路在CA1神经元中诱发兴奋性突触后电位(EPSP)-抑制性突触后电位(IPSP)序列。CNQX(10微米)大大降低了EPSP,而对输入电阻或膜电位没有任何影响。如果刺激电极放置在距离记录电极约1mm处,则IPSP也会降低。保留CNQX存在的EPSP具有NMDA受体介导的电位特征;它具有缓慢的时间过程,高频求和,被APV可逆地阻断,在无Mg2+的介质中尺寸大大增加,在含Mg2+的介质中表现出异常的电压依赖性。在CNQX的存在下,apv敏感的多突触GABAergic IPSP可被诱发,表明NMDA受体可介导抑制性中间神经元的阈上EPSPS。提示NMDA受体和非NMDA受体在不同情况下均可介导海马CA1区锥体神经元和抑制性中间神经元的突触兴奋。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Proceedings of the Royal Society of London Series B-Containing Papers of Abiological Character
Proceedings of the Royal Society of London Series B-Containing Papers of Abiological Character 生命科学, 发育生物学与生殖生物学, 发育生物学
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