[Serological classification of Bacteroides gingivalis and purification of group specific antigens].

Shika Kiso Igakkai zasshi = Japanese journal of oral biology Pub Date : 1989-12-01 DOI:10.2330/joralbiosci1965.31.647

E Ishikawa, H Kumada, T Umemoto

引用次数: 4

Abstract

Bacteroides gingivalis were serologically investigated by the immunodiffusion technique using autoclave-extracted antigens and antisera against whole cells. Fifteen strains of B. gingivalis used in this study, were classified in two distinct serological groups. Eleven strains include strain 381, ATCC 33277 were classified Group A and four strains include strain Su63 were classified in Group B. The group specific antigens from B. gingivalis strain 381 (Group A) and Su63 (Group B) were partially purified from autoclave extracts by 75% ethanol precipitation and anion exchange chromatography, and were identified as polysaccharides composed of glucose (82.3%, 23.7%), rhamnose (7.1%, 12.7%), galactose (4.9%, 18.2%), mannose (2.3%, 1.7%), glucosamine (0.4%, 28.0%) and galactosamine (3.0%, 15.3%).

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[牙龈拟杆菌的血清学分类及群体特异性抗原的纯化]。

采用免疫扩散技术，利用高压灭菌法提取抗原和抗血清对全细胞进行血清学研究。本研究中使用的15株牙龈芽孢杆菌被分为两个不同的血清学组。将菌株381、ATCC 33277等11株菌株归为A组，将菌株Su63等4株菌株归为B组。采用75%乙醇沉淀和阴离子交换层析法从高压灭菌液中提取部分龈裂菌381 (A组)和Su63 (B组)的群体特异性抗原，鉴定为葡萄糖(82.3%、23.7%)、鼠李糖(7.1%、12.7%)、半乳糖(4.9%、18.2%)、甘露糖(2.3%、1.7%)、氨基葡萄糖(0.4%、半乳糖胺(3.0%，15.3%)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Shika Kiso Igakkai zasshi = Japanese journal of oral biology

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