Platelet Rich Fibrin (PRF) : un nouveau biomatériau de cicatrisation

Abstract

The PRF (platelet rich fibrin) belongs to a new generation of platelet concentrates, with simplified modes of production and without biochemical handling of blood (heparin, EDTA, bovine thrombin, calcium chloride…). In this third article, we were interested in the immunological features of this biomaterial. Indeed, during the production of the PRF by centrifugation, the platelets are not the only cellular elements of blood to be activated: the leucocytes will also secrete cytokines in reaction to the haemostatic and inflammatory phenomena artificially induced in the centrifuged tube. We thus undertook to measure out the concentrations of five molecules within the different parts of a PRF: three pro-inflammatory cytokines (IL-1 β, IL-6 and TNf-α), an anti-inflammatory cytokine (IL-4) and a key growth promoter of the angiogenesis (VEGF). Our data are correlated with those obtained in plasma (inactivated blood) and in sera (activated blood). Our results revealed that the PRF would have the ability to regulate the inflammatory phenomena and to stimulate immune processes by chemotactism. This concept could explain the reduction of post-operative infections when PRF is used as surgical additive.