Discovery of Biomarkers for Myogenous Temporomandibular Disorders Through Salivary Metabolomic Profiling: A Pilot Study.

IF 1.9 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral & Facial Pain and Headache Pub Date : 2023-11-17 DOI:10.11607/ofph.3353

Shing Ching Khoo, Yoshinobu Shoji, Chin Hoe Teh, Nyuk Ling Ma

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引用次数: 0

Abstract

Aims: To develop a new approach to provide insights into contributing factors to the etiology and pathogenesis of temporomandibular disorders (TMDs) through discrimination of the salivary metabolomic profiling of patients with TMDs of muscular origin (ie, local myalgia) and healthy individuals.

Methods: Saliva samples from 19 patients with TMDs of muscular origin (ie, local myalgia) and 39 healthy controls were collected and identified by nuclear magnetic resonance (NMR) spectroscopy. 1H NMR spectra for all samples were acquired using a Bruker Avance-III NMR spectrometer operating at 500 MHz, and data processing was performed in TopSpin, MestreNova, SIMCA, and AMIX softwares for metabolite identification.

Results: Eight key metabolites were identified between the healthy controls and patients: L-isoleucine, methylmalonic acid, isopropanolamine, dimethyl sulfone, lactic acid, 4-ethoxyphenylacetic acid, N-acetyl alanine, and D-galactose.

Conclusions: The results of this study demonstrate that NMR-based metabolomics coupled with multivariate data analysis is a powerful method for the metabolomic profiling of patients with TMDs of muscular origin (ie, local myalgia).

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通过唾液代谢组学分析发现肌源性颞下颌疾病的生物标志物:一项初步研究。

目的:通过对肌肉源性颞下颌疾病(即局部肌痛)患者和健康个体的唾液代谢组学特征进行区分，为颞下颌疾病(TMDs)的病因和发病机制提供一种新的方法。方法:采集19例肌肉源性颞下颌关节病(即局部肌痛)患者和39例健康对照者的唾液样本，采用核磁共振(NMR)技术进行鉴定。使用工作频率为500 MHz的Bruker Avance-III型核磁共振光谱仪获取所有样品的1H NMR光谱，并在TopSpin、MestreNova、SIMCA和AMIX软件中进行数据处理，以进行代谢物鉴定。结果:在健康对照和患者之间鉴定出8种关键代谢物:l -异亮氨酸、甲基丙二酸、异丙醇胺、二甲基砜、乳酸、4-乙氧基苯乙酸、n -乙酰丙氨酸和d -半乳糖。结论:本研究结果表明，基于核磁共振的代谢组学结合多变量数据分析是一种强有力的方法来分析肌肉源性颞下颌关节痛(即局部肌痛)患者的代谢组学特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oral & Facial Pain and Headache DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

5.10

自引率

4.00%

发文量

期刊介绍： Founded upon sound scientific principles, this journal continues to make important contributions that strongly influence the work of dental and medical professionals involved in treating oral and facial pain, including temporomandibular disorders, and headache. In addition to providing timely scientific research and clinical articles, the journal presents diagnostic techniques and treatment therapies for oral and facial pain, headache, mandibular dysfunction, and occlusion and covers pharmacology, physical therapy, surgery, and other pain-management methods.