Electrochemical Immunosensor Based on Al-TCPP Nanomaterial Adsorption Aggregation Signal Amplification for the Detection of Dengue Virus NS1 Protein

IF 2.7 4区化学 Q3 CHEMISTRY, PHYSICAL

Electrocatalysis Pub Date : 2023-10-26 DOI:10.1007/s12678-023-00847-6

Zixuan Zhang, Wei Li, Jie Liu, Ying Li, Jingfu Qiu, Chaorui Li

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Abstract

To detect the dengue virus NS1 protein with high sensitivity, this research suggests an electrochemical immunosensor based on the Al-TCPP adsorption aggregation signal amplification approach. We created a type of metal-organic framework (MOF) material called astrophytum myriostigma, which resembles a cactus plant and has a large specific surface area. In addition, it can produce electrostatic attraction with the amino groups on methylene blue (MB), firmly fix MB on the MOF material, and manage MB reunion after adsorption, which is helpful for electron transmission and amplifies the electrical signal. The cationic dye methylene blue has redox characteristics. It possesses a high electron transfer rate, electrochemical reversibility, and strong biocompatibility. The generated electrochemical immunosensor has good reproducibility and stability, and the relationship between the analyte concentration and electrical signal strength is linear. The suggested immunosensor has a broad detection range from 10 fg/mL to 100 ng/mL with a low detection limit of 9.12 fg/mL under ideal conditions.

Graphical Abstract

Abstract Image

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基于 Al-TCPP 纳米材料吸附聚集信号放大技术的电化学免疫传感器用于检测登革热病毒 NS1 蛋白

为了高灵敏度地检测登革热病毒 NS1 蛋白，本研究提出了一种基于 Al-TCPP 吸附聚集信号放大方法的电化学免疫传感器。我们创造了一种名为 Astrophytum myriostigma 的金属有机框架（MOF）材料，它类似于仙人掌植物，具有很大的比表面积。此外，它还能与亚甲基蓝（MB）上的氨基产生静电吸引，将甲基蓝牢固地吸附在 MOF 材料上，并在吸附后管理甲基蓝的团聚，从而有助于电子传输并放大电信号。阳离子染料亚甲基蓝具有氧化还原特性。它具有较高的电子转移率、电化学可逆性和较强的生物相容性。生成的电化学免疫传感器具有良好的重现性和稳定性，分析物浓度与电信号强度之间呈线性关系。该免疫传感器的检测范围从 10 fg/mL 到 100 ng/mL，在理想条件下检测限低至 9.12 fg/mL。

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来源期刊

Electrocatalysis CHEMISTRY, PHYSICAL-ELECTROCHEMISTRY

CiteScore

4.80

自引率

6.50%

发文量

审稿时长

>12 weeks

期刊介绍： Electrocatalysis is cross-disciplinary in nature, and attracts the interest of chemists, physicists, biochemists, surface and materials scientists, and engineers. Electrocatalysis provides the unique international forum solely dedicated to the exchange of novel ideas in electrocatalysis for academic, government, and industrial researchers. Quick publication of new results, concepts, and inventions made involving Electrocatalysis stimulates scientific discoveries and breakthroughs, promotes the scientific and engineering concepts that are critical to the development of novel electrochemical technologies. Electrocatalysis publishes original submissions in the form of letters, research papers, review articles, book reviews, and educational papers. Letters are preliminary reports that communicate new and important findings. Regular research papers are complete reports of new results, and their analysis and discussion. Review articles critically and constructively examine development in areas of electrocatalysis that are of broad interest and importance. Educational papers discuss important concepts whose understanding is vital to advances in theoretical and experimental aspects of electrochemical reactions.