Dot-enzyme immunoassay for visual detection of Brucella melitensis antibodies.

Abstract

An indirect enzyme-linked immunosorbent assay, using antigen coupled to paper, has been adapted for the detection of Brucella melitensis antibodies. Optimum conditions were achieved by incubation of 1 ml of diluted serum with a single piece of paper coated with purified Brucella antigens for a period of one hour, and by addition of a goat anti-human enzyme conjugate antibody for one hour again. Under these conditions 80 human sera were examined and the results obtained were compared with Wright agglutination test.