Optimisation of sperm cryopreservation protocol for Asian stinging catfish Heteropneustes fossilis (Bloch, 1794), to support hatchery seed production

IF 0.4 4区农林科学 Q4 FISHERIES

Indian Journal of Fisheries Pub Date : 2023-06-30 DOI:10.21077/ijf.2023.70.2.105772-8

Kuldeep K Lal, Anindya S. Barman, Peyush Puniya, Praveen Khare, Vindhya Mohindra, Rajiv K. Singh, Rama S. Sah, Sullip K. Majhi, Bechan Lal

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引用次数: 0

Abstract

The present study experimentally evaluated different parameters such as extender composition, storage duration and egg to sperm ratio to optimise parameters for sperm cryopreservation in Asian stinging catfish Heteropneustes fossilis. Four extender compositions viz. Hanks Balanced Salt Solution (HBSS), Modified HBSS (M-HBSS), Modified HBSS with hen’s egg yolk (M-HBSS with EY) and European catfish extender were evaluated for sperm cryopreservation with dimethyl sulphoxide (DMSO) as cryoprotectant (10% v/v). The hatching rate was used as standard to evaluate the fertility of thawed sperm at different periods of storage (2, 270 and 668 days). After 2 days, extender M-HBSS exhibited highest hatching rate (49.06±2.67%) which was comparable to the control value (51.00±7.23%), followed by HBSS (42.76±32.0%), M-HBSS with EY (37.46±24.34%) and European catfish extender (29.47±25.61%), respectively. After 270 days with M-HBSS, hatching rate was 64.34±8.84% similar to the control; however, cryopreserved sperm with other extenders exhibited lower hatching rate as compared to control and M-HBSS. After 668 days, sperm cryopreserved in the extender M-HBSS, only yielded successful hatching. Results indicated that presence of egg yolk in the extender M-HBSS did not improve the performance of cryopreserved sperm. The successful hatching using cryopreserved sperm after 270 and 668 days of storage indicates M-HBSS as a useful extender for sperm cryobanking and seed production of H. fossilis. Keywords: Aquaculture, Cryopreservation, Cryoprotectant, Dimethyl sulphoxide, Extender, Hanks Balanced Salt Solution, Hatching rate

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亚洲刺鱼Heteropneustes化石精子冷冻保存方案的优化(Bloch, 1794)，以支持孵卵种子生产

本研究对亚洲刺鲶化石的精子冷冻保存参数进行了实验评价，包括延长剂的组成、保存时间、卵精比等。研究了汉克斯平衡盐溶液(HBSS)、改性HBSS (M-HBSS)、鸡蛋蛋黄改性HBSS (M-HBSS with EY)和欧洲鲶鱼增剂4种以二甲亚砜(DMSO)为冷冻保护剂(10% v/v)的精子低温保存效果。以孵化率为标准评价解冻精子在不同贮存期(2,270和668天)的育性。2 d后，M-HBSS的孵化率最高(49.06±2.67%)，与对照组(51.00±7.23%)相当，其次是HBSS(42.76±32.0%)、M-HBSS与EY(37.46±24.34%)和欧洲鲶鱼(29.47±25.61%)。经M-HBSS处理270 d，孵化率为64.34±8.84%，与对照组相似;然而，与对照组和M-HBSS相比，使用其他延长剂冷冻保存的精子的孵化率较低。668天后，精子在扩展器M-HBSS中冷冻保存，仅孵化成功。结果表明，添加卵黄的M-HBSS并没有提高精子的冷冻性能。冷冻保存270天和668天的精子成功孵化表明M-HBSS是一种有用的精子冷冻储存和种子生产的扩展剂。关键词:水产养殖，低温保存，冷冻保护剂，二甲基亚砜，扩展剂，汉克斯平衡盐溶液，孵化率

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来源期刊

Indian Journal of Fisheries FISHERIES-

CiteScore

0.90

自引率

20.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Indian Journal of Fisheries is published quarterly by the Indian Council of Agricultural Research (ICAR), New Delhi. Original contributions in the field of Fish and fisheries science are considered for publication in the Journal. The material submitted must be unpublished and not under consideration for publication elsewhere. Papers based on research which kills or damages any species, regarded as thratened/ endangered by IUCN crieteria or is as such listed in the Red Data Book appropriate to the geographic area concerned, will not be accepted by the Journal, unless the work has clear conservation objectives.