Using α -amylase enzyme with the seed pre-soaking to develop emergence and yield of triticale

Abstract

In order to investigate the efficiency of stimulating stored seeds of Triticale (X Triticosecale wittmack) by α -amylase enzyme, a field experiment was carried out using split plot arrangements according to a randomized complete block design in three replications. The storage period of seeds 6 months, 1.5 years, and 2.5 years represented the main plots. The stimulation treatments was secondary plots included dry seeds (control), stimulated seeds with distilled water, 1 and 2 mg L-1 of α -amylase enzyme. The results showed a significant decrease in the percentage of emergence, plant leaf area, plant dry weight, number of tillers m-2, number of spikes m-2, weight of one thousand grains, and grain yield with the increasing of storage period. The enzyme concentration of 1 mg L-1 was the superior in % emergence (85.02), Plant leaf area (244.88) cm2, plant dry weight (2.087) g, number of tillers (448.8) tiller m-2, number of spikes (372.9) spike m-2, number of grains spike-1 (44.13) grain spike-1 and grain yield (5.842) ton h-1. However, there was on significant differences between the two concentration 1 and 2 mg L-1 of enzyme in leaf area, plant dry weight, 1000 grain weight and grain yield.