ideal time to add Colcemid to obtain a division of the lymphocyte cells

Abstract

This research was conducted to determine the optimal conditions for the addition, and withdrawal times of colcemid by culturing colcemid in different media and to examine the effect of colcemid on lymphocyte proliferation and chromosome quality. Peripheral blood (PBL) was collected in a short-term culture. The colcemid incubation period is one factor that may affect experimental results. Routine karyotyping procedures for lymphocyte cultures (blood cultures) include exposing cells to colchicine to stop metaphase cell division and visualizing chromosomes. The exposure time to colchicine in previous research is 1 to 2 hours. After adjusting the duration of exposure to colchicine to improve chromosome quality. Although genetic/laboratory experiments were standardized by division, different durations of colchicine exposure were performed to obtain longer chromosomes with split chromosomes. We observed that shorter periods of colchicine exposure resulted in better-quality chromosomes, while longer periods of colchicine exposure resulted in fertilized chromosomes.