The effect of ethanol in the separation of wheat gliadins by CM- and DEAE-cellulose chromatography.

Abstract

The effect of ethanol on the fractionation of gliadin by cellulose-based ion exchange column chromatography was studied. Ten peaks have been separated on DEAE- and 12 peaks on CM-cellulose column using a complex elution technique. By SDS-polyacrylamide gel electrophoresis of the separated fractions, it was observed that ethanol incorporated into the eluent improved the resolution of some fractions. Some of the peaks gave only 2 or 3 protein bands in electrophoregrams. Since ethanol does not affect the reproducibility in the two types of chromatographic medium, the method seems to be suitable for purification of gliadin fractions in large quantities.