Genetic grouping of selected RILs for yield and attributing traits in determinate type of Indian bean

Abstract

The Indian bean is a well-known legume and green vegetable crop in India. An attempt was made to analyze the diversity pattern in selected recombinant inbred lines (RILs) of determinate type of Indian bean using principal component analysis (PCA) and D 2 statistics. Data from eleven yield component traits were used to carry-out the analysis. Clustering of lines was performed using modified (sequential) tocher method, and results were also compared with original tocher method. PCA analysis showed that first three principal components collectively accounted for 68.92% of total variation among the genotypes and had eigenvalues >1. The lines were grouped into five clusters using modified tocher method for cluster analysis, which has been shown to be superior to tocher method. Cluster-I and cluster-IV showed the highest inter-cluster distance (124.92) and crossing between genotypes from these two clusters was suggested to get desirable segregants. The study also suggested that PCA and D 2 analysis can be used alternatively to assess the genetic diversity pattern among genotypes.