Differential DNase I hypersensitivity of ras oncogenes in B6C3F1, C3H/He, and C57BL/6 mouse liver.

Molecular toxicology Pub Date : 1989-07-01
R L Vorce, J I Goodman
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Abstract

The male hybrid B6C3F1 mouse exhibits a 30% spontaneous hepatoma incidence, whereas the paternal C3H/He strain and the maternal C57BL/6 strain exhibit a 60% and a negligible incidence, respectively. In addition, both male and female B6C3F1 mice are extremely sensitive to chemical induction of hepatocarcinogenesis. The Ha-ras, Ki-ras, and myc oncogenes have been implicated in a variety of solid tumors. Specifically, Ha- and, less frequently, Ki-ras have been reported to be activated in B6C3F1 mouse liver tumors. The objective of this study was to examine a possible point of transcriptional control of Ha-ras, Ki-ras, and myc in all three mouse strains, our hypothesis being that these oncogenes may be primed for expression in the nascent liver of those strains exhibiting a high spontaneous hepatoma incidence. A positive correlation has been established between gene expression and the presence of DNAase I hypersensitive sites. DNase I hypersensitive sites were observed in the Ha-ras and myc oncogenes in the three mouse strains. However, Ha-ras appears to possess an additional site in B6C3F1 and C3H/He as compared to C57BL/6. Similarly, the Ki-ras oncogene exhibited a DNase I hypersensitive site only in B6C3F1 and C3H/He mouse liver. These results indicate that the hepatoma-prone strains (B6C3F1 and C3H/He) may have a greater potential for Ha- and Ki-ras expression than does the non-hepatoma-prone strain (C57BL/6).

B6C3F1、C3H/He和C57BL/6小鼠肝脏中ras癌基因的差异DNase I超敏性
雄性杂交B6C3F1小鼠的自发性肝癌发生率为30%,而父系C3H/He株和母系C57BL/6株的自发性肝癌发生率分别为60%和可忽略不计。此外,雄性和雌性B6C3F1小鼠对化学诱导肝癌发生都极为敏感。Ha-ras、Ki-ras和myc癌基因与多种实体瘤有关。具体来说,Ha-和较少出现的Ki-ras在B6C3F1小鼠肝肿瘤中被激活。本研究的目的是研究在所有三种小鼠品系中Ha-ras、Ki-ras和myc可能的转录控制点,我们的假设是这些癌基因可能在那些表现出高自发性肝癌发病率的品系的新生肝脏中被启动表达。基因表达与DNAase I超敏位点存在正相关。在3株小鼠的Ha-ras和myc癌基因中均观察到dna酶I的超敏位点。然而,与C57BL/6相比,Ha-ras似乎在B6C3F1和C3H/He中拥有一个额外的位点。同样,Ki-ras癌基因仅在B6C3F1和C3H/He小鼠肝脏中表现出DNase I超敏位点。这些结果表明,肝癌易发菌株(B6C3F1和C3H/He)可能比非肝癌易发菌株(C57BL/6)具有更大的Ha-和Ki-ras表达潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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