{"title":"Characteristics of lead adaptation in a rat kidney cell line. II. Effect on DNA synthesis, protein synthesis, and gene expression.","authors":"B Hitzfeld, D M Taylor","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The effects of adaptation of normal rat kidney cells (NRK 52-E) to growth in 5 or 10 microM lead nitrate on the rates of DNA synthesis and on the rate and pattern of protein synthesis was studied. The rate of [3H]thymidine incorporation into DNA was increased in normal cells, but remained unchanged in one lead-adapted cell line (only 5 microM NRK studied). Increased rates of [3H]leucine incorporation into nonadapted NRK cells were found only at times up to 3 h; in contrast, the lead-adapted cells showed such increases only at longer times. The most pronounced differences between normal and lead-adapted cells were found with lead concentrations of 10 or 50 microM lead nitrate. Lead-adapted control cells incorporated 170% of the [3H]leucine taken up by nonadapted cells. In both adapted and nonadapted cells the pattern of synthesis of specific proteins showed varied and dose-dependent differences between the three cell sublines examined. The changed sensitivity of both DNA and protein synthesis following lead exposure appears to be a potent parameter in the development of resistance, perhaps through the development of specific lead-binding proteins.</p>","PeriodicalId":77750,"journal":{"name":"Molecular toxicology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular toxicology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The effects of adaptation of normal rat kidney cells (NRK 52-E) to growth in 5 or 10 microM lead nitrate on the rates of DNA synthesis and on the rate and pattern of protein synthesis was studied. The rate of [3H]thymidine incorporation into DNA was increased in normal cells, but remained unchanged in one lead-adapted cell line (only 5 microM NRK studied). Increased rates of [3H]leucine incorporation into nonadapted NRK cells were found only at times up to 3 h; in contrast, the lead-adapted cells showed such increases only at longer times. The most pronounced differences between normal and lead-adapted cells were found with lead concentrations of 10 or 50 microM lead nitrate. Lead-adapted control cells incorporated 170% of the [3H]leucine taken up by nonadapted cells. In both adapted and nonadapted cells the pattern of synthesis of specific proteins showed varied and dose-dependent differences between the three cell sublines examined. The changed sensitivity of both DNA and protein synthesis following lead exposure appears to be a potent parameter in the development of resistance, perhaps through the development of specific lead-binding proteins.