Characteristics of lead adaptation in a rat kidney cell line. II. Effect on DNA synthesis, protein synthesis, and gene expression.

Molecular toxicology Pub Date : 1989-07-01
B Hitzfeld, D M Taylor
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Abstract

The effects of adaptation of normal rat kidney cells (NRK 52-E) to growth in 5 or 10 microM lead nitrate on the rates of DNA synthesis and on the rate and pattern of protein synthesis was studied. The rate of [3H]thymidine incorporation into DNA was increased in normal cells, but remained unchanged in one lead-adapted cell line (only 5 microM NRK studied). Increased rates of [3H]leucine incorporation into nonadapted NRK cells were found only at times up to 3 h; in contrast, the lead-adapted cells showed such increases only at longer times. The most pronounced differences between normal and lead-adapted cells were found with lead concentrations of 10 or 50 microM lead nitrate. Lead-adapted control cells incorporated 170% of the [3H]leucine taken up by nonadapted cells. In both adapted and nonadapted cells the pattern of synthesis of specific proteins showed varied and dose-dependent differences between the three cell sublines examined. The changed sensitivity of both DNA and protein synthesis following lead exposure appears to be a potent parameter in the development of resistance, perhaps through the development of specific lead-binding proteins.

大鼠肾细胞系对铅的适应特性。2对DNA合成、蛋白质合成和基因表达的影响。
研究了正常大鼠肾细胞(NRK 52-E)在5和10 μ m硝酸铅中生长的适应性对其DNA合成速率、蛋白质合成速率和模式的影响。正常细胞中[3H]胸苷并入DNA的速率增加,但在一种铅适应细胞系中保持不变(仅研究了5微米NRK)。[3H]亮氨酸掺入非适应性NRK细胞的比率仅在3小时内增加;相比之下,适应铅的细胞仅在较长时间内表现出这种增长。正常细胞和铅适应细胞之间最明显的差异是在铅浓度为10或50微米硝酸铅时发现的。铅适应对照细胞吸收了未适应细胞吸收的170%的[3H]亮氨酸。在适应和非适应细胞中,特定蛋白质的合成模式在所检查的三种细胞亚系之间表现出不同的剂量依赖性差异。铅暴露后DNA和蛋白质合成敏感性的变化似乎是耐药性发展的一个有力参数,可能是通过特定铅结合蛋白的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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