Characteristics of lead adaptation in a rat kidney cell line. I. Uptake and subcellular and subnuclear distribution of lead.

Molecular toxicology Pub Date : 1989-07-01
B Hitzfeld, D M Taylor
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Abstract

Two sublines of normal rat kidney cell (NRK 52-E) that are resistant to 5 and 10 microM lead nitrate have been developed and characterized. The cellular and nuclear uptake of 210Pb, as well as the subnuclear distribution, was unchanged by lead adaptation: it remained at values of 2.3-6.6 pmole 210Pb/microgram protein/h. Also no difference in the slope of the curve of the uptake rate of the three cell types could be detected. Studies of the nuclear uptake of 210Pb showed that in both normal and lead-adapted cells the major lead fraction was associated with the chromatin and mostly (63-67%) with the nuclear proteins. Electron microscopic studies demonstrated that particulate lead was taken up in all lead-exposed cells. It was concluded that development of resistance to lead does not arise from a reduced rate of cellular uptake, from increased excretion, or from changes in nuclear uptake or in the subnuclear distribution within the cells.

大鼠肾细胞系对铅的适应特性。铅的摄取和亚细胞和亚核分布。
研究了正常大鼠肾细胞NRK 52-E对5和10 μ m硝酸铅的耐药亚系。铅适应后,细胞和细胞核对210Pb的摄取以及亚核分布没有变化,保持在2.3-6.6摩尔210Pb/微克蛋白/小时。三种细胞摄取速率曲线的斜率也无差异。对210Pb核摄取的研究表明,在正常细胞和铅适应细胞中,铅的主要部分与染色质有关,大部分(63-67%)与核蛋白有关。电子显微镜研究表明,微粒铅在所有铅暴露的细胞中被吸收。结论是,对铅的抗性的发展不是由于细胞摄取率的降低、排泄量的增加或细胞内核摄取或亚核分布的变化引起的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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