Evaluation of Antimicrobial Effects of Fresh and Dried Fruit in Turkish Tombul Hazelnut Variety

Seda BİRYOL, Mehtap USTA, Ali SOYDİNÇ
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 Materials and Methods: The Turkish Tombul hazelnuts used in the experiment were obtained from Helvacı Village/ Düzköy /Trabzon. Fresh hazelnuts were harvested in 2023, while dried hazelnuts were collected in 2022. The dried hazelnuts were dried at 105°C until they attained a consistent weight. This section was not created for fresh hazelnuts. The treated micelle was extracted for 4 hours with 100 mL of Ethyl alcohol (EtOH) for every 10 g of both the fresh and dried hazelnut samples crushed. The micelle (hazelnut+oil+solvent mixture) was filtered through coarse filter paper to remove particles before being blown away with a Rotary evaporator (Heidolph, Germany). The resulting extracts were used for antimicrobial testing. Antimicrobial activity of the strains was evaluated against Pseudomonas aeruginosa ATCC 27853, Enterobacter cloaceae ATCC2468, Enterococcus feacalis ATCC51299, Escherichia coli ATCC2471, Klebsiella pneumoniae ATCC700603, Salmonella typhimurium ATCC13311, Serretia marcescens ATCC13880, Staphylococcus epidermis ATCC14990, Staphylococcus aureus ATCC25923, Bacillus subtilis ATCC 6633, Streptococcus faecalis ATCC 9790, Yersinia pestis ATCC 19428 and Candida albicans ATCC10351. One-way analysis of variance (ANOVA) was run to determine any significant differences in the study groups by Duncan multiple range test was performed through SPSS.
 Results: Dried hazelnut extract was effective in all of them, while fresh hazelnut extract was only effective on Candida albicans ATCC10351 and Klebsiella pneumoniae ATCC700603 (F (3, 8) = 65.45, p","PeriodicalId":7545,"journal":{"name":"Akademik Ziraat Dergisi","volume":"12 2","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Akademik Ziraat Dergisi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29278/azd.1355422","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract

Objective: The aim of the study was to determine the antimicrobial properties of fresh and dried fruits of Turkish Tombul hazelnut (Corylus avellana L.) variety. Materials and Methods: The Turkish Tombul hazelnuts used in the experiment were obtained from Helvacı Village/ Düzköy /Trabzon. Fresh hazelnuts were harvested in 2023, while dried hazelnuts were collected in 2022. The dried hazelnuts were dried at 105°C until they attained a consistent weight. This section was not created for fresh hazelnuts. The treated micelle was extracted for 4 hours with 100 mL of Ethyl alcohol (EtOH) for every 10 g of both the fresh and dried hazelnut samples crushed. The micelle (hazelnut+oil+solvent mixture) was filtered through coarse filter paper to remove particles before being blown away with a Rotary evaporator (Heidolph, Germany). The resulting extracts were used for antimicrobial testing. Antimicrobial activity of the strains was evaluated against Pseudomonas aeruginosa ATCC 27853, Enterobacter cloaceae ATCC2468, Enterococcus feacalis ATCC51299, Escherichia coli ATCC2471, Klebsiella pneumoniae ATCC700603, Salmonella typhimurium ATCC13311, Serretia marcescens ATCC13880, Staphylococcus epidermis ATCC14990, Staphylococcus aureus ATCC25923, Bacillus subtilis ATCC 6633, Streptococcus faecalis ATCC 9790, Yersinia pestis ATCC 19428 and Candida albicans ATCC10351. One-way analysis of variance (ANOVA) was run to determine any significant differences in the study groups by Duncan multiple range test was performed through SPSS. Results: Dried hazelnut extract was effective in all of them, while fresh hazelnut extract was only effective on Candida albicans ATCC10351 and Klebsiella pneumoniae ATCC700603 (F (3, 8) = 65.45, p
土耳其榛子品种鲜果和干果抗菌效果评价
目的:研究土耳其黑榛(Corylus avellana L.)鲜果和干果的抗菌性能。材料和方法:实验中使用的土耳其Tombul榛子来自helvaci Village/ Düzköy /Trabzon。新鲜榛子于2023年收获,而干榛子于2022年收获。干榛子在105°C下干燥,直到它们达到一致的重量。这部分不是为新鲜榛子创建的。用100 mL乙醇(EtOH)对每10 g粉碎的新鲜和干燥榛子样品提取处理过的胶束4小时。胶束(榛子+油+溶剂混合物)通过粗滤纸过滤去除颗粒,然后用旋转蒸发器(Heidolph,德国)吹走。所得提取物用于抗菌试验。测定菌株对铜绿假单胞菌atcc27853、阴肠杆菌ATCC2468、粪肠球菌ATCC51299、大肠杆菌ATCC2471、肺炎克雷伯菌ATCC700603、鼠伤寒沙门氏菌ATCC13311、粘质丝孢杆菌ATCC13880、表皮葡萄球菌ATCC14990、金黄色葡萄球菌ATCC25923、枯草芽孢杆菌atcc6633、粪链球菌atcc9790、鼠疫杆菌atcc19428和白色念珠菌ATCC10351的抑菌活性。采用单因素方差分析(ANOVA),通过SPSS进行Duncan多元极差检验,以确定各研究组之间是否存在显著差异;结果:干榛子提取物对两组患者均有效,而鲜榛子提取物仅对白色念珠菌ATCC10351和肺炎克雷伯菌ATCC700603有效(F (3,8) = 65.45, p
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