Phylloremediation of pyrene and anthracene by endophytic fungi inhabiting tea leaves ( Camellia sinensis (L.) Kuntze) in Sri Lanka

IF 0.8 4区生物学 Q4 PLANT SCIENCES

New Zealand Journal of Botany Pub Date : 2023-09-20 DOI:10.1080/0028825x.2023.2258829

Lanka Undugoda, Kasun Thambugala, Sagarika Kannangara, Jayantha Munasinghe, Nadeeka Premarathna, Nadeema Dharmasiri

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引用次数: 0

Abstract

ABSTRACTPolyaromatic hydrocarbons (PAHs) released from vehicular emissions and oil refineries deposit on the phyllosphere, compromising the quality of leaf-based food products by posing many health issues. Nevertheless, the tea phyllosphere harbours a variety of endophytes that are highly effective at degrading polyaromatic hydrocarbons, anthracene, and pyrene. The present study attempts to analyse the pyrene and anthracene degrading capability of phyllosphere endophytic fungi that inhabit Camellia sinensis (L.) Kuntze leaves. The frequency of occurrence of endophytic fungi in different leaf tissue layers was examined using light and scanning electron microscopy (SEM). The best pyrene and anthracene degrading strains were selected based on the High-Performance Liquid Chromatography (HPLC) results, and further kinetic assays. Light microscopy and SEM observations highlighted a heterogeneous endophytic fungal distribution among leaf tissue layers; the upper epidermis had the highest fungal distribution compared to other leaf layers. HPLC results revealed that Phyllosticta capitalensis, Colletotrichum gloeosporioides, Colletotrichum siamense, Pseudopestalotiopsis chinensis, and Daldinia eschscholtzii, have higher pyrene and anthracene degradation respectively and their PAH degradation kinetics follow the first-order kinetic model. The best anthracene and pyrene degrader, P. capitalensis showed the lowest half-life. The present investigation highlights the potential of P. capitalensis, the best pyrene and anthracene degrader that can remediate PAHs deposited on the phyllosphere of tea leaves.KEYWORDS: Bioremediationfungal endophyteskineticspollutantspolyaromatic hydrocarbons (PAHs) AcknowledgmentsThe authors wish to acknowledge the Department of Plant and Molecular Biology, University of Kelaniya, Sri Lanka, for providing facilities to carry out this research component.Disclosure statementNo potential conflict of interest was reported by the author(s).

查看原文本刊更多论文

茶树内生真菌对芘和蒽的层状修复作用Kuntze)在斯里兰卡

摘要汽车尾气和炼油厂排放的多环芳烃(PAHs)沉积在叶层圈中，造成许多健康问题，影响叶基食品的质量。然而，茶叶层球中含有多种内生菌，这些内生菌在降解多芳烃、蒽和芘方面非常有效。本文分析了茶树叶根圈内生真菌对芘和蒽的降解能力。Kuntze树叶。利用光镜和扫描电镜(SEM)研究了不同叶组织层内生真菌的发生频率。通过高效液相色谱(HPLC)和动力学分析筛选出芘和蒽的最佳降解菌株。光镜和扫描电镜观察显示叶片组织层间内生真菌分布不均;与其他叶层相比，上表皮真菌分布最多。HPLC结果显示，毛竹(Phyllosticta capitalensis)、炭疽菌(Colletotrichum gloeosporioides)、siamense炭疽菌(Pseudopestalotiopsis chinensis)和Daldinia eschscholtzii对芘和蒽的降解均较高，且其对PAH的降解动力学符合一级动力学模型。对蒽和芘的降解效果最好的是capitalensis，半衰期最短。本研究结果表明，白杨是修复茶叶叶层中多环芳烃的最佳芘和蒽降解剂。关键词:生物修复;真菌内生动力学;污染;多芳烃(PAHs)致谢作者感谢斯里兰卡克拉尼亚大学植物与分子生物学系为开展本研究提供了设施。披露声明作者未报告潜在的利益冲突。

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来源期刊

New Zealand Journal of Botany 生物-植物科学

CiteScore

2.20

自引率

22.20%

发文量

审稿时长

>12 weeks

期刊介绍： The New Zealand Journal of Botany publishes original research papers, review papers, perspectives, short communications, forum articles, letter and book reviews. We welcome submissions relevant to all aspects of the botany, mycology, and phycology of the South Pacific, Australia, South America, and Southern Africa. The journal’s subject matter encompasses biosystematics and biogeography, ecology, physiology, biochemistry, genetics, reproductive biology, structure and development, taxonomy, ethnobotany, palaeobotany, bryology, lichenology, mycology, plant pathology, and phycology.