Evaluation of Platelet Storage Lesion (PSL) Markers in SSP+ Platelet Additive Solution Added Canine Platelet Concentrates (CPC)

Abstract

Background: To evaluate the Platelet Storage Lesion (PSL) markers and extended storage time for SSP+ added Canine Platelet Concentrates (CPC) beyond five days at 22°C.Fresh Canine CPCundergo certain metabolic and morphologic changes during storage. Estimation of PSL markers helps us better understand quality of the canine PC and to estimate the efficacy of SSP+ in increasing the life span of stored CPC. Method:CPC was prepared from 350 ml of whole blood, collected from 6 apparently healthy donor dogs by buffy coat methodin a quadruple bag closed system. CPC was divided into four aliquots of 15 ml each with one control and three test groups with SSP+ additive solution in different concentrations (65%, 75%, 85%). TheIn vitro PSL markers including swirling, pH, glucose, lactate, bicarbonate, pO2, pCO2, platelet concentration, MPV and PDWwere evaluated on day 1, 5, 9 and 13 of storage. Result:The results were analysed by Two-way ANOVA wherein swirling, pH, glucose, lactate, bicarbonate, pO2, pCO2, platelet concentration, MPV showed significant difference (p less than 0.01) between the groups. Interaction between the additive concentrations and the days of storage revealed a pH on day 9 for 65% SSP+ to be similar to day 5 for Plasma stored CPC while swirling, lactate and bicarbonate were also better maintained for 65% SSP+ PAS added CPC till day 9.Addition of 65% SSP+ PAS to CPC evidenced increased shelf life up to 9 days at 22°C under agitation without significant deterioration in product quality.