Cerium-based demonstration of phosphatase activity in plastic-embedded sections: a comparison with conventional methods.

H van Goor, K Poelstra, M J Hardonk
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引用次数: 6

Abstract

Cerium-based methods have been used for the demonstration of several phosphatases at alkaline, acid and neutral pH in low temperature acetone-fixed, plastic-embedded sections. At alkaline pH calcium is used as capturing agent and the precipitated calcium phosphate converted to cerium phosphate. At neutral and acid pH cerium is used directly as capturing agent. Cerium phosphate is subsequently visualized using the H2O2-DAB method. A comparison has been made with conventional calcium-cobalt and lead methods. It appeared that calcium-cobalt methods are more susceptible to improvement than lead methods.

塑料包埋切片中磷酸酶活性的铈基演示:与常规方法的比较。
在低温丙酮固定、塑料包埋切片中,基于铈的方法已被用于几种磷酸酶在碱性、酸性和中性pH下的演示。在碱性条件下,钙作为捕集剂,沉淀的磷酸钙转化为磷酸铈。在中性和酸性pH下,铈直接用作捕集剂。随后使用H2O2-DAB方法可视化磷酸铈。并与传统的钙钴法和铅法进行了比较。钙钴法似乎比铅法更容易改进。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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