Bioactive compounds of Citrullus colocynthis aerial parts: characterization and biological properties

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-09-30 DOI:10.1080/11263504.2023.2257720

Ammar B. Altemimi, Muthanna J. Mohammed, Zina T. Alkanan, Shaymaa H. Abdulrahman, Shatha A. Allaitha, Tarek Gamal Abedelmaksoud, Qausar Hamed ALKaisy, Mazin A. A. Najm, Francesco Cacciola, P. Vasantha Srinivasan

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Abstract

AbstractIn this study, the bioactive components of Citrullus colocynthis extracts were analyzed in view of their potential use as antioxidant/antibacterial agent. The bioactive compounds were extracted in different organic solvents (hexane, ethanol and ethyl acetate) and analyzed by column chromatography, thin-layer chromatography, high-performance liquid chromatography and gas chromatography-mass spectrophotometry (GC-MS). Three distinct fractions were collected and subsequently examined, one from ethyl acetate extraction (I) and two from ethanolic extraction (II and III). p-Coumaric acid was the major phenolic acid from the ethyl acetate extracts (0.11 ± 0.023 mg/kg) while resorcinol was the major phenolic compound from the ethanol extracts (25.00 ± 3.21 mg/kg). Twenty-four bioactive compounds were identified by GC-MS analysis where 1-docosanol pentafluoropropionate was the major bioactive compound in ethanol extracts. Among the ethanol extracts, the highest antioxidant activity was found in fraction III. The highest anti-bacterial activity values, measured in terms of the size of the inhibition zone (mm) against Bacillus cereus and Staphylococcus aureus, i.e. 24 ± 1.62 and 22 ± 2.19 mm, respectively, were attained at 24 µg/mL for fractions III and I. From the results achieved in this work, ethanol extracts of C. colocynthis aerial parts could be considered promising as antioxidant and anti-infectious agents.Keywords: Citrullus colocynthis aerial partsbioactive compoundshPLCGC-msantioxidantantibacterial Authors’ contributionAmmar B. Altemimi: Conceptualization, Writing—Original draft, Project administration. Muthanna J. Mohammed: Investigation. Zina T. Alkanan: Investigation. Shaymaa H. Abdulrahman, Investigation. Shatha A. Allaitha: Investigation. Tarek Gamal Abedelmaksoud: Writing—Reviewing and editing, Supervision. Qausar Hamed ALKaisy: Investigation. Qausar Hamed ALKaisy: Investigation. Mazin A. A. Najm: Writing—Reviewing and editing. Francesco Cacciola: Conceptualization, Writing—Reviewing and editing, Supervision. P. Vasantha Srinivasan: Writing—Reviewing and editing, Supervision.Disclosure statementThe authors declare no conflict of interest.

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甜瓜地上部位的生物活性化合物:表征及生物学特性

摘要本研究分析了香瓜提取物的生物活性成分，探讨了其作为抗氧化/抗菌药物的潜力。在不同的有机溶剂(己烷、乙醇和乙酸乙酯)中提取生物活性化合物，并采用柱层析、薄层析、高效液相色谱和气相色谱-质谱联用(GC-MS)分析。分别从乙酸乙酯提取液(ⅰ)和乙醇提取液(ⅱ和ⅲ)中提取3个不同的组分，乙酸乙酯提取液中主要的酚类化合物为对香豆酸(0.11±0.023 mg/kg)，乙醇提取液中主要的酚类化合物为间苯二酚(25.00±3.21 mg/kg)。通过气相色谱-质谱分析鉴定出24种生物活性化合物，其中1-二十二醇五氟丙酸是乙醇提取物中的主要生物活性化合物。在乙醇提取物中，抗氧化活性最高的部位为ⅲ。对蜡样芽孢杆菌和金黄色葡萄球菌的抑菌区大小(mm)测定表明，在24µg/mL浓度下，三组分和一组分的抑菌区大小分别为24±1.62和22±2.19 mm，具有较高的抑菌活性。关键词:甜瓜;空气成分;生物活性化合物;Muthanna J. Mohammed:调查。吉娜:调查。Shaymaa H. Abdulrahman，调查。Shatha A. Allaitha:调查。Tarek Gamal Abedelmaksoud:写作-审查和编辑，监督。Qausar Hamed ALKaisy:调查。Qausar Hamed ALKaisy:调查。Mazin A. A. Najm:写作-评论和编辑。Francesco Cacciola:概念化，写作审查和编辑，监督。P. Vasantha Srinivasan:写作-审查和编辑，监督。声明作者声明无利益冲突。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.