Evaluating the impact of organic matter and sample processing techniques on RNA detection using environmental samples

IF 0.7 4区农林科学 Q3 Agricultural and Biological Sciences

Journal of Swine Health and Production Pub Date : 2023-03-01 DOI:10.54846/jshap/1311

Grace Houston, Allison Blomme, Olivia Harrison, Jianfa Bai, Jason Woodworth, Cassandra Jones, Elizabeth Poulsen-Porter, Chad Paulk, Jordan Gebhardt

引用次数: 0

Abstract

This study evaluated sample processing methods and the presence of organic matter on detection of porcine epidemic diarrhea virus (PEDV) from environmental samples using real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). Steel coupons were inoculated with PEDV and different types of organic material contamination. Surface samples were collected and processed in one of four ways: none, centrifugation, syringe filtration, or combination of centrifugation and syringe filtration, then submitted for PEDV qRT-PCR. There was a surface inoculation type by processing method interaction (P < .001) that impacted the sample cycle threshold value. Centrifugation resulted in the most consistent detection of PEDV RNA.

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评估有机物和样品处理技术对使用环境样品进行RNA检测的影响

采用实时逆转录-聚合酶链式反应(qRT-PCR)技术对环境样品中猪流行性腹泻病毒(PEDV)的检测方法及有机物含量进行了评价。钢片分别接种PEDV和不同类型的有机物污染。收集表面样品并采用以下四种方法之一进行处理:无、离心、注射器过滤或离心和注射器过滤联合处理，然后提交PEDV qRT-PCR。加工方法相互作用形成表面接种型(P <.001)，影响样本周期阈值。离心法对PEDV RNA的检测结果最为一致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Swine Health and Production 农林科学-兽医学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： The Journal of Swine Health & Production (JSHAP) is an open-access and peer-reviewed journal published by the American Association of Swine Veterinarians (AASV) since 1993. The aim of the journal is the timely publication of peer-reviewed papers with a scope that encompasses the many domains of applied swine health and production, including the diagnosis, treatment, management, prevention and eradication of swine diseases, welfare & behavior, nutrition, public health, epidemiology, food safety, biosecurity, pharmaceuticals, antimicrobial use and resistance, reproduction, growth, systems flow, economics, and facility design. The journal provides a platform for researchers, veterinary practitioners, academics, and students to share their work with an international audience. The journal publishes information that contains an applied and practical focus and presents scientific information that is accessible to the busy veterinary practitioner as well as to the research and academic community. Hence, manuscripts with an applied focus are considered for publication, and the journal publishes original research, brief communications, case reports/series, literature reviews, commentaries, diagnostic notes, production tools, and practice tips. All manuscripts submitted to the Journal of Swine Health & Production are peer-reviewed.