Characterization of regional proteins in tissues and fluids in the human epididymis.

H R Junera, M F Alfonsi, M A Fain-Maurel, J P Dadoune
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Abstract

The proteins of epididymal tissues and fluids recovered from five regions of the human epididymis were separated by polyacrylamide gel electrophoresis under denaturing conditions. Among the 60 peptides identified, eight appeared to be expressed solely in the epididymal duct when compared to serum and testis proteins. Three of these (92, 47 and 24 Kd) showed a degree of regional specificity in fluids. The 92 Kd peptide was found in the caput and proximal corpus, the 47 Kd in the distal corpus and cauda and the 24 Kd in the caput of the epididymis. Three of the specific epididymal proteins (39, 30, 26 Kd) displayed a remarkable analogy to those found in man and monkey in other conditions and which are present at the sperm surface in the epididymis cauda.

人附睾组织和体液中区域蛋白的表征。
在变性条件下,用聚丙烯酰胺凝胶电泳法分离了从人附睾5个部位提取的附睾组织和液体的蛋白质。在鉴定的60个肽中,与血清和睾丸蛋白相比,8个肽似乎仅在附睾管中表达。其中3个(92,47和24kd)在液体中显示一定程度的区域特异性。在附睾头部和近端发现92 Kd肽,在远端和尾部发现47 Kd肽,在附睾头部发现24 Kd肽。其中三种特异性附睾蛋白(39,30,26kd)与人类和猴子在其他条件下发现的蛋白质非常相似,它们存在于附睾尾部的精子表面。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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