Use of polyvinyl alcohol-based cationic hydrogels modified with gold nanoparticles as drug and gene delivery systems with enhanced antibacterial properties

IF 2.1 3区 化学 Q3 POLYMER SCIENCE
İrem Simge Goregen, Ozgur Ozay
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引用次数: 0

Abstract

AbstractIn this study, PVA/p(HEMA-co-MAPTAC) hydrogels were synthesized by redox polymerization using polyvinyl alcohol (PVA), 2-hydroxyethyl methacrylate (HEMA) and [3-(Methacryloylamino)propyl]trimethylammonium chloride (MAPTAC). In order to increase the antimicrobial activities of synthesized PVA/p(HEMA-co-MAPTAC) hydrogels, they were modified with Au nanoparticles. The swelling behavior of the synthesized hydrogels was investigated. Hydrogels were characterized by various techniques. The drug release studies of PVA/p(HEMA-co-MAPTAC) and PVA/p(HEMA-co-MAPTAC)@Au hydrogels were performed in PBS medium using Ribonucleic acid (RNA) as genetic material and sodium diclofenac (NaDc) as drug. It was determined that PVA/p(HEMA-co-MAPTAC) hydrogel held 91.5 mg/g RNA and 97.48 mg/g NaDc and released 28.32 mg/g RNA and 71.12 mg/g NaDc in PBS medium. Subsequently, PVA/p(HEMA-co-MAPTAC)@Au hydrogel adsorbed 87.46 mg/g RNA and 119.44 mg/g NaDc and released 18.96 mg/g RNA, 104.99 mg/g NaDc drug in PBS medium. Using the data obtained at the end of the release studies, the drug release kinetics of the hydrogels were examined. According to the Korsmeyer Peppas model, RNA release from hydrogels showed Fickian diffusion, while NaDc release showed non-Fickian diffusion behavior. The antibacterial and antifungal activities of the hydrogels were tested using gram-negative bacteria E. coli, gram-positive bacteria B. subtilis and a fungus species C. albicans.Keywords: Hydrogelpolyvinyl alcoholdrug releasegold nanoparticleantibacterial activity AcknowledgmentThis study includes a part of the master thesis of Irem Simge Goregen, and the authors thank Çanakkale Onsekiz Mart University Science and Technology Application and Research Center (COBILTUM) for analyses.Disclosure statementNo potential conflict of interest was reported by the authors.
用金纳米颗粒修饰的聚乙烯醇基阳离子水凝胶作为增强抗菌性能的药物和基因传递系统
摘要以聚乙烯醇(PVA)、甲基丙烯酸2-羟乙基(HEMA)和[3-(甲基丙烯胺)丙基]三甲基氯化铵(MAPTAC)为原料,采用氧化还原聚合法制备了PVA/p(HEMA-co-MAPTAC)水凝胶。为了提高合成的PVA/p(HEMA-co-MAPTAC)水凝胶的抗菌活性,对其进行了纳米金修饰。研究了合成的水凝胶的溶胀行为。水凝胶通过各种技术进行表征。以核糖核酸(RNA)为遗传物质,双氯芬酸钠(NaDc)为药物,在PBS培养基中进行PVA/p(HEMA-co-MAPTAC)和PVA/p(HEMA-co-MAPTAC)@Au水凝胶的药物释放研究。测定PVA/p(HEMA-co-MAPTAC)水凝胶在PBS培养基中含有91.5 mg/g RNA和97.48 mg/g NaDc,释放28.32 mg/g RNA和71.12 mg/g NaDc。随后,PVA/p(HEMA-co-MAPTAC)@Au水凝胶吸附87.46 mg/g RNA和119.44 mg/g NaDc,在PBS培养基中释放18.96 mg/g RNA和104.99 mg/g NaDc药物。利用释放研究结束时获得的数据,检查了水凝胶的药物释放动力学。根据Korsmeyer Peppas模型,水凝胶中的RNA释放表现为菲克式扩散,而NaDc释放表现为非菲克式扩散。用革兰氏阴性菌大肠杆菌、革兰氏阳性菌枯草芽孢杆菌和真菌白念珠菌对水凝胶的抗菌和抗真菌活性进行了测试。本研究为Irem Simge Goregen硕士论文的一部分,作者感谢Çanakkale Onsekiz Mart大学科技应用与研究中心(COBILTUM)的分析。披露声明作者未报告潜在的利益冲突。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.60
自引率
16.00%
发文量
61
审稿时长
3.1 months
期刊介绍: Journal of Macromolecular Science, Part A: Pure and Applied Chemistry (JMS-PAC) is a necessary resource for academic and industrial scientists and engineers whose interests center on both synthetic and naturally occurring polymers and their applications.
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