Inactivation of Avian Influenza Virus Aerosol Using Membrane-Less Electrolyzed Water Spraying

Shinhao Yang, Chi-Yu Chuang, Hsiao-Chien Huang, Wei Fang
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Abstract

Avian influenza virus (AIV) can have a serious impact on both human and animal health. In this study, we used an environmentally controlled chamber and a hemagglutination assay (HA) to evaluate the ability of membrane-less electrolyzed water (MLEW) spraying to inactivate H6N1 AIV aerosol. MLEW was generated by electrolyzing sodium chloride solution, and then sprayed into the chamber at free available chlorine (FAC) concentrations of 50, 100 and 200 mg/L by means of high-pressure air pumping to inactivate airborne H6N1 AIV aerosols individually. The results showed that MLEW spraying effectively neutralized H6N1 AIV aerosol. In addition, the virucidal ability of MLEW increased as the FAC concentration increased. Five minutes after MLEW spraying at an FAC concentration of 200 mg/L, the H6N1 viral HA titer decreased from 24 to 20. Our work provides important new evidence of the value of spraying with MLEW disinfectant to protect against AIV, which may be further applied for indoor decontamination purposes to promote animal and human health.
无膜电解水喷雾法灭活禽流感病毒气雾剂
禽流感病毒(AIV)可对人类和动物健康产生严重影响。在这项研究中,我们使用环境控制室和血凝试验(HA)来评估无膜电解水(MLEW)喷雾灭活H6N1 AIV气溶胶的能力。通过电解氯化钠溶液生成MLEW,然后通过高压气泵将游离有效氯(FAC)浓度分别为50、100和200 mg/L的气体喷射到实验室内,分别灭活空气中的H6N1 AIV气溶胶。结果表明,MLEW喷雾能有效中和H6N1 AIV气溶胶。此外,随着FAC浓度的增加,MLEW的杀病毒能力增强。FAC浓度为200 mg/L的MLEW喷施5 min后,H6N1病毒HA滴度由24降至20。本研究提供了重要的新证据,证明了MLEW消毒剂喷洒对AIV的防护价值,可进一步应用于室内净化目的,以促进动物和人类的健康。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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