Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.)

Q4 Biochemistry, Genetics and Molecular Biology
Nevena Nagl, Lovro Sinkovič, Aleksandra Savić, Milada Isakov, Hourieh Hasanaklou, Barbara Pipan, Ana Marjanović-Jeromela
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引用次数: 0

Abstract

Grass pea (Lathyrus sativus) is a valuable grain legume known for its high protein content and rich essential amino acid profile. Its exceptional characteristics such as drought tolerance, high adaptability to extreme conditions, disease resistance, and low cultivation inputs, make it particularly suitable for cultivation by resource-poor farmers. However, the potential use of grass pea is limited due to the presence of anti-nutritional factors, including protease inhibitors, especially trypsin inhibitor. This study aimed to develop a rapid and reliable method for measuring TI activity in seeds of grass pea and to investigate the influence of genotype and environment on trypsin inhibitor (TI) activity in seeds of grass pea. A set of 25 grass pea accessions from seven European countries was cultivated in Slovenia at the Agricultural Institute of Slovenia, and a set of 12 grass pea accessions from the Serbian gene bank was grown in Serbia at the Institute of Field and Vegetable Crops. The TI activity varied considerably among the grass pea accessions studied, with values ranging from 26.7 to 90.3 TUI/mg. To further evaluate the effects of environmental conditions on TI activity, eight grass pea accessions originating from Serbia were grown in both Slovenia and Serbia. The TI activity of the accessions grown in Slovenia ranged from 26.7 to 81.0 TUI/mg, while the activity of the accessions grown in Serbia ranged from 40.3 to 57.0 TUI/mg. The correlation of TI activity between grass pea accessions grown in Slovenia and those grown in Serbia was 0.39, with genotype diversity being the largest contributor (55.9%). This study provides a valuable insight into the variability of TI activity in grass pea and shows the possible influence of environmental conditions on this trait. However, since the data are only from a one-year field trial, further studies are needed to fully evaluate the influence of different environmental factors on TI activity.
草豆种子胰蛋白酶抑制剂活性的研究
草豆(Lathyrus sativus)是一种有价值的谷物豆科植物,以其高蛋白含量和丰富的必需氨基酸而闻名。它具有耐旱性、对极端条件的高适应性、抗病性和低栽培投入等特殊特性,特别适合资源贫乏的农民种植。然而,由于存在抗营养因子,包括蛋白酶抑制剂,特别是胰蛋白酶抑制剂,草豆的潜在用途受到限制。本研究旨在建立一种快速、可靠的测定草豆种子中胰蛋白酶抑制剂(TI)活性的方法,探讨基因型和环境对草豆种子中胰蛋白酶抑制剂(TI)活性的影响。斯洛文尼亚农业研究所在斯洛文尼亚种植了来自七个欧洲国家的25个草豆品种,塞尔维亚大田和蔬菜作物研究所在塞尔维亚种植了来自塞尔维亚基因库的12个草豆品种。不同草豆材料TI活性差异较大,为26.7 ~ 90.3 TUI/mg。为了进一步评估环境条件对TI活性的影响,研究人员在斯洛文尼亚和塞尔维亚分别种植了8种原产于塞尔维亚的草豌豆。斯洛文尼亚生长的材料TI活性在26.7 ~ 81.0 TUI/mg之间,塞尔维亚生长的材料TI活性在40.3 ~ 57.0 TUI/mg之间。斯洛文尼亚草豆与塞尔维亚草豆TI活性的相关系数为0.39,其中基因型多样性对TI活性的影响最大(55.9%)。该研究为草豆TI活性的变异性提供了有价值的见解,并显示了环境条件对该性状的可能影响。然而,由于数据仅来自为期一年的野外试验,需要进一步的研究来充分评估不同环境因素对TI活性的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Ratarstvo i Povrtarstvo
Ratarstvo i Povrtarstvo Agricultural and Biological Sciences-Soil Science
CiteScore
0.80
自引率
0.00%
发文量
4
审稿时长
4 weeks
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