Changes in membrane lipid order by microplasma irradiation for high molecule drug absorption

Q3 Physics and Astronomy

Plasma Medicine Pub Date : 2023-01-01 DOI:10.1615/plasmamed.2023050304

Sadia Afrin Rimi, Jaroslav Kristof, Md Jahangir Alam, Oishi kyohei, Okada Takumi, Abubakar Hamza Sadiq, Shimizu Kazuo

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引用次数: 0

Abstract

Plasma irradiation for drug delivery has attracted significant attention due to its advantages over conventional delivery methods. However, the underlying mechanism of plasma-mediated drug delivery remains largely unexplored. Cell membranes are mainly composed of lipid bilayer which can be easily affected by plasma components, such as reactive species. However, the effects of microplasma irradiation on cell membrane lipids have not been studied before. Therefore, the current study investigated the changes in the lipid packing of cell membranes after microplasma irradiation. In this study, we first optimized the conditions for high molecular weight drug (fluorescein isothiocyanate-dextran, Mw:150 kDa and 2000 kDa) absorption into intestinal epithelial cells. Air microplasma discharge at several voltages (3.5 kV, 4 kV, and 4.5 kV) was applied, and drug absorption was confirmed using a fluorescence microscopy and microplate reader. The best results were achieved at 4 kV in terms of both drug absorption and cell viability. Next, the lipid packing of the membrane was investigated in microplasma irradiated cells (at 4 kV) using lipiORDER dye. The results showed that the membrane lipid order has changed from liquid order phase to liquid disorder phase due to plasma irradiation. The findings of the current study will help understanding the mechanism of cellular drug absorption using microplasma irradiation.

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微等离子体辐照对高分子药物吸收膜脂序的影响

等离子体辐照给药由于其相对于传统给药方法的优越性而受到广泛关注。然而，血浆介导的药物传递的潜在机制在很大程度上仍未被探索。细胞膜主要由脂质双分子层组成，极易受到活性物质等血浆组分的影响。然而，微等离子体辐照对细胞膜脂质的影响尚未见研究。因此，本研究探讨了微等离子体辐照后细胞膜脂质堆积的变化。在本研究中，我们首先优化了高分子量药物(异硫氰酸荧光素-葡聚糖，分子量:150 kDa和2000 kDa)在肠上皮细胞中的吸收条件。施加不同电压(3.5 kV、4 kV和4.5 kV)的空气微等离子体放电，并使用荧光显微镜和微孔板阅读器确认药物吸收。在4 kV时，药物吸收和细胞活力均达到最佳。接下来，在微等离子体照射细胞(4kv)中，使用lipiORDER染料研究膜的脂质包装。结果表明，等离子体辐照使膜脂由液体有序相转变为液体无序相。本研究结果将有助于理解微等离子体辐照对细胞药物吸收的机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plasma Medicine Physics and Astronomy-Physics and Astronomy (all)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Technology has always played an important role in medicine and there are many journals today devoted to medical applications of ionizing radiation, lasers, ultrasound, magnetic resonance and others. Plasma technology is a relative newcomer to the field of medicine. Experimental work conducted at several major universities, research centers and companies around the world over the recent decade demonstrates that plasma can be used in variety of medical applications. It is already widely used surgeries and endoscopic procedures. It has been shown to control properties of cellular and tissue matrices, including biocompatibility of various substrates. Non-thermal plasma has been demonstrated to deactivate dangerous pathogens and to stop bleeding without damaging healthy tissue. It can be used to promote wound healing and to treat cancer. Understanding of various mechanisms by which plasma can interact with living systems, including effects of reactive oxygen species, reactive nitrogen species and charges, has begun to emerge recently. The aim of the Plasma Medicine journal will be to provide a forum where the above topics as well as topics closely related to them can be presented and discussed. Existing journals on plasma science and technology are aimed for audiences with primarily engineering and science background. The field of Plasma Medicine, on the other hand, is highly interdisciplinary. Some of prospective readers and contributors of the Plasma Medicine journal are expected to have background in medicine and biology. Others might be more familiar with plasma science. The goal of the proposed Plasma Medicine journal is to bridge the gap between audiences with such different backgrounds, without sacrificing the quality of the papers be their emphasis on medicine, biology or plasma science and technology.