Optimization of the TRV-based Gene Silencing Efficiency and Growth Condition on Solanum lycopersicum var. ‘Hawaii7996’ Resistant to Bacterial Wilt Disease

IF 1 4区 农林科学 Q3 HORTICULTURE
Nayoung Kim, Won-Hee Kang, Ji-Su Kwon, Hyo-Jin Kim, Boseul Park, Seon-In Yeom
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引用次数: 0

Abstract

Ralstonia solanacearum (R. sol), a pathogen that causes wilt symptoms, is widely distributed in many regions. Breeding plants resistant against this pathogen is well known as the most effective control method. To this end, it is necessary to reveal the function of resistance genes, and virus- induced gene-silencing (VIGS) technology is one of the most widely used methods for analyzing gene functions. However, the problem with functional analysis of bacterial wilt resistance genes based on VIGS is the conflicting temperature environments of inoculated strains. Therefore, the objective of this study is to identify optimal plant size and temperature conditions for experiments using two srains considering a less complicated functional analysis of R. sol resistant candidate genes. We applied five different temperatures and then assessed the disease progress of R. sol inoculated tomatoes. The plants with the highest VIGS efficiency were those treated at 22°C for four weeks (4L), three weeks at a low temperature and one week at 30°C (3L1H), and two weeks at 22°C and two weeks at 30°C (2L2H). Also, we confirmed the most suitable temperature for identifying candidate genes against R. sol was found to be 22°C for plants grown for three weeks, followed by 30°C for one week. The results of this study can provide useful data to those seeking to establish a foundation for functional analysis research focusing on genes related to tomato bacterial wilt resistance.
抗青枯病番茄品种‘夏威夷7996’trv基因沉默效率及生长条件的优化
茄枯病菌(Ralstonia solanacearum, R. sol)是一种引起青枯病的病原菌,广泛分布在许多地区。培育对该病菌具有抗性的植物是众所周知的最有效的防治方法。为此,有必要揭示抗性基因的功能,而病毒诱导基因沉默(VIGS)技术是目前应用最广泛的基因功能分析方法之一。然而,基于VIGS的抗青枯病基因功能分析存在接种菌株温度环境冲突的问题。因此,本研究的目的是考虑到对土壤抗性候选基因不太复杂的功能分析,确定两个菌株实验的最佳植株大小和温度条件。采用5种不同温度对接种番茄进行了病害防治。VIGS效率最高的植物是在22°C条件下处理4周(4L),在30°C条件下处理3周(3L1H),在22°C和30°C条件下处理2周(2L2H)。结果表明,22°C是培养3周的最佳温度,30°C是培养1周的最佳温度。本研究结果可为番茄抗青枯病相关基因的功能分析研究奠定基础。
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来源期刊
CiteScore
2.00
自引率
0.00%
发文量
0
审稿时长
1 months
期刊介绍: Horticultural Science and Technology (abbr. Hortic. Sci. Technol., herein ‘HST’; ISSN, 1226-8763), one of the two official journals of the Korean Society for Horticultural Science (KSHS), was launched in 1998 to provides scientific and professional publication on technology and sciences of horticultural area. As an international journal, HST is published in English and Korean, bimonthly on the last day of even number months, and indexed in ‘SCIE’, ‘SCOPUS’ and ‘CABI’. The HST is devoted for the publication of technical and academic papers and review articles on such arears as cultivation physiology, protected horticulture, postharvest technology, genetics and breeding, tissue culture and biotechnology, and other related to vegetables, fruit, ornamental, and herbal plants.
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