Molecular and morphological characterization of three novel Minorisa species (Chlorarachnea) and proposal for an emended description of the Minorisa minuta

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-10-17 DOI:10.1111/pre.12533

Takashi Shiratori, Yuta Kato, Ken‐ichiro Ishida

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引用次数: 1

Abstract

SUMMARY Minorisa is a group of tiny uniflagellates belonging to the Cercozoa. These flagellates are the closest heterotrophic relatives of photosynthetic chlorarachniophytes and are one of the most abundant bacterivorous eukaryotes in coastal marine environments. Despite their evolutionary and ecological importance, taxonomic studies on Minorisa have not been conducted since the original description. In the present study, we isolated five Minorisa strains and performed molecular phylogenetic and microscopic analyses. Molecular phylogenetic analysis using small subunit ribosomal RNA gene sequences indicated that the strains form four different subclades in Minorisa . Microscopic observations revealed that these Minorisa strains possess an amoeboid stage that lacks a flagellum. In the amoeboid stage, cells possess lobose and/or extrusive pseudopodia. Based on the phylogenetic analysis and morphological observations, we revised the description of Minorisa and described the following three novel Minorisa species: Minorisa fusiformis sp. nov., Minorisa magna sp. nov. and Minorisa megafusiformis sp. nov.

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三种小虾属(Chlorarachnea)的分子和形态特征及小虾属(Minorisa minuta)的修订建议

小鞭毛纲是一组微小的单鞭毛纲动物，属于尾虫纲。这些鞭毛虫是光合绿蛛纲植物最近的异养亲戚，是沿海海洋环境中最丰富的细菌真核生物之一。尽管它们在进化和生态学上具有重要意义，但从最初的描述开始，对米诺瑞沙的分类研究就没有进行过。在本研究中，我们分离了5株Minorisa菌株并进行了分子系统发育和显微分析。利用小亚基核糖体RNA基因序列进行的分子系统发育分析表明，该菌株在Minorisa中形成了四个不同的亚支系。显微镜观察显示，这些Minorisa菌株具有一个变形虫阶段，缺乏鞭毛。在变形虫阶段，细胞具有叶状和/或挤压的假足。在系统发育分析和形态学观察的基础上，我们对该属植物的描述进行了修正，确定了3个新种:Minorisa fusiformis sp. nov.、Minorisa magna sp. nov.和Minorisa megafusiformis sp. nov.。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.