Interference with the distribution and release of arachidonic acid in human keratinocytes by bradykinin, histamine and phosphatidic acid

Abstract

The effects of bradykinin, histamine, phosphatidic acid and leukotrienes B₄ and C₄ on the distribution and release of ¹⁴C-arachidonic acid in human keratinocytes in culture were investigated. Bradykinin, histamine, and phosphatidic acid were found to liberate ¹⁴C-arachidonic acid from membrane phospholipids, whereas leukotrienes B₄ and C₄ were ineffective in this respect. The decrease in the labeling of phospholipids was accompanied by increased labeling of the non-phosphorus lipids. The present study suggests that bradykinin, histamine, and phosphatidic acid may interfere with the distribution and release of arachidonic acid in human keratinocytes in culture.