Evaluation of immunological response to hepatitis B vaccine among healthcare workers at Rwanda Military Hospital

Q4 Medicine

Rwanda Medical Journal Pub Date : 2023-10-13 DOI:10.4314/rmj.v80i3.8

C. Muhinda, J. Kabahizi, K. Bazatsinda, N. Dukuze, C. Nsanzabaganwa, G. Murenzi, M. Yotebieng, B. Bagaya, L. Mutesa

{"title":"Evaluation of immunological response to hepatitis B vaccine among healthcare workers at Rwanda Military Hospital","authors":"C. Muhinda, J. Kabahizi, K. Bazatsinda, N. Dukuze, C. Nsanzabaganwa, G. Murenzi, M. Yotebieng, B. Bagaya, L. Mutesa","doi":"10.4314/rmj.v80i3.8","DOIUrl":null,"url":null,"abstract":"      INTRODUCTION: Healthcare workers (HCWs) are at high risk of acquiring hepatitis B viral (HBV) infection through occupational exposure to blood or body fluids. However, the rates of non- responders after HBV vaccination among HCWs are not well documented. Therefore, we aimed to determine the proportion of immunological non-responders among HCWs who received Hepatitis B vaccine at Rwanda Military Hospital (RMH) and characterize the memory T cell responses to the Hepatitis B vaccine. METHODS: A cross sectional study was conducted at the RMH. HBV vaccinated HCWs were evaluated for immune response by measuring serum Hepatitis B surface antibody (anti-HBs) titers and levels of HB core antibodies (HBcAb) on COBAS e411 machine, 6 years post vaccination.RESULTS: Initially 87 employees were included in the study. Four participants were excluded due to incomplete records. 52 (62.7%) participants were female and 31 (37.3%) were male. HWCs’ age ranged between 23 and 66 years with a mean (±SD) age of 38.2 ± 7.3. Of 83 HCWs, 70 (84.3%)showed response to HBV vaccine, non-responders were 11 (13.3%) and 2 (2.4%) showed immunity due to natural infection. There was no significant difference in Th cell frequencies and function between responders and non-responders after stimulation with rHBsAg vaccine. CONCLUSION: Immunological response six years post HBsAg vaccination was 84.3% in HCWs at RMH and similar to the global prevalence. Anti-HBs levels should be tested in all HCWs following HBsAg vaccination. Personal protective equipment, and a dose of Hepatitis B prophylaxis when exposed should be emphasized.     ","PeriodicalId":38181,"journal":{"name":"Rwanda Medical Journal","volume":"7 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rwanda Medical Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/rmj.v80i3.8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

INTRODUCTION: Healthcare workers (HCWs) are at high risk of acquiring hepatitis B viral (HBV) infection through occupational exposure to blood or body fluids. However, the rates of non- responders after HBV vaccination among HCWs are not well documented. Therefore, we aimed to determine the proportion of immunological non-responders among HCWs who received Hepatitis B vaccine at Rwanda Military Hospital (RMH) and characterize the memory T cell responses to the Hepatitis B vaccine. METHODS: A cross sectional study was conducted at the RMH. HBV vaccinated HCWs were evaluated for immune response by measuring serum Hepatitis B surface antibody (anti-HBs) titers and levels of HB core antibodies (HBcAb) on COBAS e411 machine, 6 years post vaccination.RESULTS: Initially 87 employees were included in the study. Four participants were excluded due to incomplete records. 52 (62.7%) participants were female and 31 (37.3%) were male. HWCs’ age ranged between 23 and 66 years with a mean (±SD) age of 38.2 ± 7.3. Of 83 HCWs, 70 (84.3%)showed response to HBV vaccine, non-responders were 11 (13.3%) and 2 (2.4%) showed immunity due to natural infection. There was no significant difference in Th cell frequencies and function between responders and non-responders after stimulation with rHBsAg vaccine. CONCLUSION: Immunological response six years post HBsAg vaccination was 84.3% in HCWs at RMH and similar to the global prevalence. Anti-HBs levels should be tested in all HCWs following HBsAg vaccination. Personal protective equipment, and a dose of Hepatitis B prophylaxis when exposed should be emphasized.

查看原文本刊更多论文

卢旺达军事医院医护人员对乙肝疫苗免疫反应的评价

& # x0D;& # x0D;& # x0D;& # x0D;& # x0D;& # x0D;导读:卫生保健工作者(HCWs)通过职业接触血液或体液感染乙型肝炎病毒(HBV)的风险很高。然而，在卫生保健工作者中，乙肝疫苗接种后无应答的比率并没有很好的记录。因此，我们的目的是确定在卢旺达军事医院(RMH)接受乙肝疫苗的医护人员中免疫无应答者的比例，并描述记忆T细胞对乙肝疫苗的反应。方法:在RMH进行横断面研究。接种乙肝疫苗后6年，在COBAS e411机器上检测血清乙型肝炎表面抗体(anti-HBs)滴度和乙肝核心抗体(HBcAb)水平，评价接种乙肝疫苗后的免疫应答。结果:最初有87名员工被纳入研究。4名受试者因记录不完整而被排除。女性52例(62.7%)，男性31例(37.3%)。年龄在23 ~ 66岁之间，平均(±SD)年龄为38.2±7.3岁。83例HCWs中，70例(84.3%)对HBV疫苗有应答，11例(13.3%)无应答，2例(2.4%)因自然感染产生免疫。rHBsAg疫苗刺激后，应答者和无应答者的细胞频率和功能无显著差异。结论:乙肝表面抗原接种6年后的免疫应答率为84.3%，与全球流行率相似。接种乙肝表面抗原后，所有卫生保健工作者都应检测抗乙肝病毒水平。应强调个人防护装备，并在接触乙肝病毒时采取一定剂量的预防措施。& # x0D;& # x0D;& # x0D;& # x0D;& # x0D;

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Rwanda Medical Journal Medicine-Medicine (all)

CiteScore

0.20

自引率

0.00%

发文量

审稿时长

7 weeks

期刊介绍： The Rwanda Medical Journal (RMJ), is a Not-For-Profit scientific, medical, journal that is published entirely online in open-access electronic format. The RMJ is an interdisciplinary research journal for publication of original work in all the major health disciplines. Through a rigorous process of evaluation and peer review, The RMJ strives to publish original works of high quality for a diverse audience of healthcare professionals. The Journal seeks to deepen knowledge and advance scientific discovery to improve the quality of care of patients in Rwanda and internationally.