RNA Viral Communities Are Structured by Host Plant Phylogeny in Oak and Conifer Leaves

IF 3.3 3区 生物学 Q2 MICROBIOLOGY
Anneliek Maria ter Horst, Jane D. Fudyma, Aurélie Bak, Min Sook Hwang, Christian Santos-Medellin, Kristian Stevens, David Rizzo, Maher Al Rwahnih, Joanne B. Emerson
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Abstract

Wild plants can suffer devastating diseases, experience asymptomatic persistent infections, and serve as reservoirs for viruses of agricultural crops; however, we have a limited understanding of the natural plant virosphere. To access representatives of locally and globally distinct wild plants and investigate their viral diversity, we extracted and sequenced double-stranded RNA from leaves from 16 healthy oak and conifer trees in the University of California–Davis Arboretum (Davis, CA, U.S.A.). From de novo assemblies, we recovered 389 RNA-dependent RNA polymerase gene sequences from 384 putative viral species, and identified 580 putative viral contigs via virus prediction software followed by manual confirmation of virus annotation. Based on similarity to known viruses, most recovered viruses were predicted to infect plants or fungi, with the highest diversity and abundance observed in the Totiviridae and Mitoviridae families. Phyllosphere viral community composition differed significantly by host plant phylogeny, suggesting the potential for host-specific viromes. The phyllosphere viral community of one oak tree differed substantially from other oak viral communities and contained a greater proportion of putative mycoviral sequences, potentially due to the tree's more advanced senescence at the time of sampling. These results suggest that oak and conifer trees harbor a vast diversity of viruses with as-yet-unknown roles in plant health and phyllosphere microbial ecology. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
橡树和针叶树叶片中基于寄主植物系统发育的RNA病毒群落
野生植物可能遭受毁灭性的疾病,经历无症状的持续感染,并成为农作物病毒的储存库;然而,我们对天然植物病毒圈的了解有限。为了获得本地和全球独特野生植物的代表并研究其病毒多样性,我们从加州大学戴维斯植物园(Davis, CA, usa)的16棵健康橡树和针叶树的叶子中提取双链RNA并对其进行测序。从从头组装中,我们从384个假定的病毒物种中恢复了389个RNA依赖的RNA聚合酶基因序列,并通过病毒预测软件鉴定了580个假定的病毒组合,然后手工确认病毒注释。根据与已知病毒的相似性,大多数恢复的病毒预计会感染植物或真菌,其中Totiviridae和Mitoviridae科的多样性和丰度最高。不同寄主植物系统发育的层球病毒群落组成差异显著,表明存在寄主特异性病毒群的可能性。一棵橡树的层球病毒群落与其他橡树的病毒群落有很大的不同,并且含有更大比例的假定的分枝病毒序列,这可能是由于该树在采样时更早衰老。这些结果表明,橡树和针叶树含有大量的病毒,这些病毒在植物健康和层际微生物生态中所起的作用尚不清楚。[公式:见正文]版权所有©2022作者。这是一篇在CC BY-NC-ND 4.0国际许可下发布的开放获取文章。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.40
自引率
6.80%
发文量
42
审稿时长
4 weeks
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