{"title":"Characterization of monoclonal antibodies directed against the TSH receptor, as revealed with the cytochemical bioassay.","authors":"N J Marshall, L D Kohn, P A Ealey","doi":"10.1530/acta.0.114s173","DOIUrl":null,"url":null,"abstract":"<p><p>The monoclonal antibody 11E8 (Kohn et al. 1984) is a novel proof of the actions of thyroid stimulator, since it is not only a potent stimulator of thyroid cells but also an inhibitor for TSH-binding. This might reflect an interaction of 11E8 with a discrete domaine of the TSH receptor, which is an essential component for TSH but not for TSab actions. These unique properties of 11E8 can be applied to the characterization of other thyroid stimulators which may interact with different domaines of the receptor. Moreover, 'TSab-like' stimulators, such as the sheep anti-TSH antiserum, could potentially be identified by 11E8.</p>","PeriodicalId":6931,"journal":{"name":"Acta endocrinologica. Supplementum","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.114s173","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta endocrinologica. Supplementum","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1530/acta.0.114s173","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
The monoclonal antibody 11E8 (Kohn et al. 1984) is a novel proof of the actions of thyroid stimulator, since it is not only a potent stimulator of thyroid cells but also an inhibitor for TSH-binding. This might reflect an interaction of 11E8 with a discrete domaine of the TSH receptor, which is an essential component for TSH but not for TSab actions. These unique properties of 11E8 can be applied to the characterization of other thyroid stimulators which may interact with different domaines of the receptor. Moreover, 'TSab-like' stimulators, such as the sheep anti-TSH antiserum, could potentially be identified by 11E8.
单克隆抗体11E8 (Kohn et al. 1984)是甲状腺刺激剂作用的新证据，因为它不仅是甲状腺细胞的强效刺激剂，而且是tsh结合的抑制剂。这可能反映了11E8与TSH受体的离散结构域的相互作用，该结构域是TSH的重要组成部分，而不是TSab的作用。11E8的这些独特性质可以应用于其他可能与受体不同结构域相互作用的甲状腺刺激物的表征。此外，“类似tsab”的刺激物，如绵羊抗tsh抗血清，可能被11E8识别。
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