Long non‑coding RNA PEG13 regulates endothelial cell senescence through the microRNA‑195/IRS1 axis

IF 2.4 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Qin Huang, Haiwen Zhou, Songping Yu
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Abstract

Atherosclerosis is a chronic inflammatory disease characterized by endothelial dysfunction and plaque formation. The present study aimed to elucidate the pathological role of the long non‑coding RNA (lncRNA) paternally expressed 13 (PEG13) in the onset and progression of atherosclerosis. Specifically, its effects on human umbilical vein endothelial cell (HUVEC) proliferation, angiogenesis, senescence and senescence‑associated secretory phenotype (SASP)‑related factors were investigated using cell proliferation, cellular angiogenesis, β‑galactosidase staining, reverse transcription‑quantitative PCR and enzyme‑linked immunosorbent assays. The results showed that oxidized low‑density lipoprotein (ox‑LDL) inhibited lncRNA PEG13 expression and HUVEC viability in a dose‑dependent manner and PEG13 overexpression partially reversed these effects. Additionally, PEG13 overexpression ameliorated the ox‑LDL‑induced impairment of angiogenesis, cellular senescence and SASP. Furthermore, lncRNA PEG13 directly targeted microRNA (miR/miRNA)‑195‑5p, suppressing the ox‑LDL‑induced upregulation of the miRNA. The gene coding for insulin receptor substrate 1 (IRS1), an activator of the phosphoinositide 3‑kinase (PI3K)/protein kinase B (AKT) signaling pathway, was confirmed as a direct target of miR‑195. PEG13 overexpression attenuated the ox‑LDL‑induced inhibition of IRS1 expression and PI3K/AKT signaling and its protective effects on HUVEC viability, angiogenesis and senescence were partially reversed by small interfering RNAs targeting IRS1. The present study demonstrated that lncRNA PEG13 attenuates ox‑LDL‑induced senescence in HUVECs by modulating the miR‑195/IRS1/PI3K/AKT signaling pathway, suggesting a potential therapeutic target for the treatment of atherosclerosis.
长链非编码RNA PEG13通过microRNA - 195/IRS1轴调控内皮细胞衰老
动脉粥样硬化是一种以内皮功能障碍和斑块形成为特征的慢性炎症性疾病。本研究旨在阐明长链非编码RNA (lncRNA)父本表达13 (PEG13)在动脉粥样硬化发生和发展中的病理作用。具体而言,采用细胞增殖、细胞血管生成、β -半乳糖苷酶染色、逆转录定量PCR和酶联免疫吸附法研究其对人脐静脉内皮细胞(HUVEC)增殖、血管生成、衰老和衰老相关分泌表型(SASP)相关因子的影响。结果表明,氧化低密度脂蛋白(ox - LDL)以剂量依赖的方式抑制lncRNA PEG13表达和HUVEC活力,PEG13过表达部分逆转了这些作用。此外,PEG13过表达可改善ox - LDL诱导的血管生成、细胞衰老和SASP损伤。此外,lncRNA PEG13直接靶向microRNA (miR/miRNA) - 195 - 5p,抑制ox - LDL诱导的miRNA上调。胰岛素受体底物1 (IRS1)的基因编码是磷酸肌肽3激酶(PI3K)/蛋白激酶B (AKT)信号通路的激活剂,被证实是miR - 195的直接靶点。PEG13过表达减弱了ox - LDL诱导的对IRS1表达和PI3K/AKT信号的抑制,其对HUVEC活力、血管生成和衰老的保护作用被靶向IRS1的小干扰rna部分逆转。本研究表明,lncRNA PEG13通过调节miR - 195/IRS1/PI3K/AKT信号通路,减轻了ox - LDL诱导的HUVECs衰老,提示了治疗动脉粥样硬化的潜在治疗靶点。
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来源期刊
Experimental and therapeutic medicine
Experimental and therapeutic medicine MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
1.50
自引率
0.00%
发文量
570
审稿时长
1 months
期刊介绍:
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