Prevalence of gastrointestinal parasites in endangered captive Asian Elephants (Elephas maximus) of Chitwan National Park in Nepal

Abstract

In order to ascertain the comprehensive prevalence of gastrointestinal parasites among captive Asian elephants in Chitwan National Park, a cross-sectional investigation was conducted. A total of 103 samples was purposefully collected. Demographic details encompassing age and gender, along with epidemiological information concerning deworming status, timing intervals, and nutritional condition for both government-owned and privately-owned elephants, were procured through a structured questionnaire survey. The process involved microscopic identification and quantification of gastrointestinal parasites through sedimentation, centrifugal floatation, and MacMaster Egg Per Gram (EPG) count methods. The resultant data indicated an overall prevalence of gastrointestinal parasites at 47.57% (49 out of 103 samples). The dominant class of parasites observed was Nematodes (n=30, 61.22%), followed by Trematodes (n=14, 28.57%) and Cestodes (n=5, 10.20%). Six distinct parasite genera were identified with positive results: Strongylus (26.53%), Trichostrongylus (24.48%), Fasciola (16.35%), Paramphistomum (12.24%), Anoplocephala (10.20%), and Ascaris (10.20%). Notably, the prevalence was markedly higher in females (39.80%) in comparison to males (7.76%), with the disparity being statistically significant (p>0.05). Additionally, a noteworthy correlation was observed between parasite prevalence, age groups, and deworming history, with statistical significance (p<0.05). The Egg Per Gram (EPG) count analysis demonstrated that the majority (87.75%) of the positively identified samples exhibited mild infection (100-500 eggs), while a relatively low percentage (6.12%) displayed heavy infection (1000-1500 eggs). The mean EPG was calculated as (248.39 ± 54.25). Consequently, the heightened prevalence of gastrointestinal parasites in captive elephants within Chitwan National Park underscores the necessity for targeted interventions to mitigate the risk of parasitic infestations.