Determination of Pneumococcal Serotypes by Sequetyping and Sequential Conventional Multiplex PCR in the Vaccine Era

IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY

Pertanika Journal of Tropical Agricultural Science Pub Date : 2023-10-31 DOI:10.47836/pjtas.46.4.10

Nurul Asyikin Abdul Rahman, Mohd Nasir Mohd Desa, Siti Norbaya Masri, Niazlin Mohd Taib, Nurshahira Sulaiman, Nurul Diana Dzaraly, Hazmin Hazman

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引用次数: 0

Abstract

Pneumococcal serotyping is required for epidemiological surveillance to guide vaccination strategy. DNA-based approaches are more affordable, but the combination of sequetyping and sequential conventional multiplex polymerase chain reaction (cmPCR) may complement one another. A total of 101 isolates were subjected to sequetyping and sequential cmPCR following previously published protocols, and the outputs were compared. The sequetyping method determined up to the serotype level for 99 isolates (98%). On the other hand, the sequential cmPCR technique identified 91 isolates (90.1%), with 63 of them (62.4%) up to the serotype level. Sequetyping generated discrete serotypes for 6A/B, 11A/D, 15A/F, and 15B/C as 6A (n = 11), 6B (n = 10), 11A (n = 5), 15C (n = 1), and 15A (n = 1). In conclusion, the cpsB gene sequetyping method produced a comparable output with sequential cmPCR, further discriminating some sub-serogroups among the isolate collection.

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序列分型和序列常规多重PCR测定疫苗时代肺炎球菌血清型

流行病学监测需要肺炎球菌血清分型，以指导疫苗接种战略。基于dna的方法更经济实惠，但序列分型和顺序传统多重聚合酶链反应(cmPCR)的结合可能互补。根据先前发表的方案，对101株分离株进行序列分型和序列cmPCR，并对输出结果进行比较。序列分型方法测定99株(98%)血清型水平。序列cmPCR技术鉴定出91株(90.1%)，其中63株(62.4%)血清型水平符合要求。序列分型可获得6A/B、11A/D、15A/F和15B/C的离散血清型，分别为6A (n = 11)、6B (n = 10)、11A (n = 5)、15C (n = 1)和15A (n = 1)。综上所述，cpsB基因序列分型方法与序列cmPCR的输出结果相当，进一步区分了分离物收集中的一些亚血清群。

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来源期刊

Pertanika Journal of Tropical Agricultural Science Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.10

自引率

16.70%

发文量