ESSENTIAL OIL COMPOSITION AND ANTIOXIDANT, ANTICANCER ACTIVITIES OF ARTEMISIA FRIGIDA WILLD. GROWN IN MONGOLIA

Irekhbayar Jambal, Amarjargal Ayurzana, Altantsetseg Shatar
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Abstract

Medicinal plants have always been considered a healthy source of life for all people. In Mongolia, the use of various medicinal and food plants has a long history. In recent years, interest in plant-derived food additives has grown. This study was designed to determine essential oil chemical composition and evaluate antioxidant, cytotoxic activities of aerial parts serial fractions from Artemisia frigida Willd. grown in Mongolia. The chemical composition of essential oil from A.frigida Willd. was determined by GC-MS analysis. Among them 40 components (35.07%), 1,8-cineol (27.22%), borneol (3.01%), terpin-4-ol (4.51%), γ-cadinene (7.5%), α-terpineol (2.98%), bornylacetate (1.80%) were found to be the major ones. The ethanol crude extract of A.frigida Willd. was suspended in water and consequently fractionated with n-hexane, chloroform, ethyl acetate and butyl alcohol. All the fractions were examined for their antioxidant and cytotoxic activities by using DPPH and MTT assays, respectively. Ethyl acetate fraction showed the highest DPPH radical scavenging activity at a concentration of 50 µg/ml by 93.17% with the IC50 value of 11.68 µg/ml. MTT results showed that water fraction demonstrated significant cytotoxicity against A549 - human alveolar basal epithelial cell line, with values of 78.69 and 60.98% (50; 10 µg/ml; at 48 h). These suggest that the fractions of A.frigida Willd. could hold a good potential for use in the pharmaceutical industry.
冷蒿精油成分及抗氧化、抗癌活性研究。生长在蒙古
药用植物一直被认为是所有人健康的生命之源。在蒙古,使用各种药用和食用植物有着悠久的历史。近年来,人们对植物性食品添加剂的兴趣日益浓厚。本研究旨在测定冷蒿空中部位系列组分的挥发油化学成分,并评价其抗氧化和细胞毒活性。生长在蒙古。冷椿精油的化学成分。GC-MS分析测定。其中,1,8-桉叶油醇(27.22%)、冰片油(3.01%)、松品-4-醇(4.51%)、γ-癸二烯(7.5%)、α-松油醇(2.98%)、龙骨乙酸酯(1.80%)为主要成分(35.07%)。冷椿的乙醇粗提物。悬浮在水中,然后用正己烷、氯仿、乙酸乙酯和丁醇进行分馏。采用DPPH法和MTT法检测各组分的抗氧化活性和细胞毒活性。乙酸乙酯部位在浓度为50µg/ml时对DPPH自由基的清除率最高,为93.17%,IC50值为11.68µg/ml。MTT结果显示,水组分对A549 -人肺泡基底上皮细胞具有显著的细胞毒性,分别为78.69%和60.98% (50;10µg / ml;这些结果表明,野生冷冻菌的部分。在制药工业中具有良好的应用潜力。
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