Effect of Canagliflozin Alone and in Combination with Metformin on Ovarian Histology of a Polycystic Ovary Syndrome Rat Model

IF 0.8 4区医学 Q4 TROPICAL MEDICINE

Biomedica Pub Date : 2023-06-25 DOI:10.24911/biomedica/5-895

Akfish Zaheer, Rabab Miraj, Sidra Mushtaq, None Qurat-tul-Ain, Hafiz Muhammad Imran Aziz, Sadia Chiragh

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Abstract

Background and Objective: Canagliflozin reduces insulin resistance in diabetics and is hypothesized to produce a beneficial effect in polycystic ovarian syndrome (PCOS). Therefore, this study is planned to compare the effects of canagliflozin and metformin alone and in combination on ovarian histology of rat models with letrozole-induced PCOS. Methods: It was a randomized experimental study on Sprague Dawley rats. A total of N = 40 female rats were divided randomly into six groups (A-F). With the exception of normal control group A, rats were given letrozole 1 mg/kg daily for 21 days till PCOS was induced. Group B was disease control, while rats in groups C-F were administered canagliflozin (10 mg/kg), metformin (100 mg/kg), a combination of canagliflozin (10 mg/kg) with metformin (100 mg/kg), and combination of canagliflozin (5 mg/kg) with metformin (50 mg/kg), respectively. Animals were sacrificed on the 48th day. Ovaries and uterus were removed, weighed, and processed for further histopathological analysis. Results: All treatment groups showed significant improvement in ovarian histology. The number of primary and secondary follicles and cystic follicles was significantly lower (p < 0.001) in all treatment groups as compared to the disease control group. Conclusion: Canagliflozin is effective for the treatment of PCOS and augments the effect of metformin in a rat model.

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卡格列净单用及联用二甲双胍对多囊卵巢综合征大鼠卵巢组织学的影响

< 背景和目标:卡格列净降低糖尿病患者的胰岛素抵抗，并被假设对多囊卵巢综合征(PCOS)产生有益作用。因此，本研究拟比较卡格列净和二甲双胍单用和联用对来曲唑诱导PCOS大鼠模型卵巢组织学的影响。 & lt; p> & lt; strong>方法:& lt; / strong>这是一项对斯普拉格·道利大鼠的随机实验研究。N = 40只雌性大鼠随机分为6组(A- f)。除正常对照组A外，其余大鼠给予来曲唑1 mg/kg / d，连续21 d至PCOS诱导。B组为疾病对照组，C-F组大鼠分别给予卡格列净(10 mg/kg)、二甲双胍(100 mg/kg)、卡格列净(10 mg/kg)与二甲双胍(100 mg/kg)联用、卡格列净(5 mg/kg)与二甲双胍(50 mg/kg)联用。动物在第48天被宰杀。切除卵巢和子宫，称重，并进行进一步的组织病理学分析。 & lt; p> & lt; strong>结果:& lt; / strong>各治疗组卵巢组织学均有明显改善。原发性、继发性卵泡和囊性卵泡数量明显减少(p <0.001)，所有治疗组与疾病对照组相比。 & lt; p> & lt; strong>结论:& lt; / strong>在大鼠模型中，加格列净可有效治疗多囊卵巢综合征，并可增强二甲双胍的作用。

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来源期刊

Biomedica 医学-热带医学

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biomédica is the quarterly journal of the Instituto Nacional de Salud of Colombia [Colombias National Health Institute]. Its purpose is to publish the results of original research that contributes meaningfully to knowledge in health and biomedical sciences.