"New Perspectives on Technical Aspects of Micromanipulation (ICSI) in Clinical Assisted Reproduction"

Abstract

Intracytoplasmic Sperm Injection (ICSI) is presently performed for 70-80% of the ART (assisted reproductive technology) cycles [1]. This substantial use is partly due to the excessive stage of standardization through the procedure. The aim of this review was to consider the available publications dealing with the technical aspect of ICSI in assisted reproduction. The literature search was conducted employing PubMed and Google scholar. Some of the key words used: ICSI injection, ICSI outcomes, sperm immobilization, micro tools, commercial micropipette, home-made micropipette, injection speed, injection force, aspiration of cytoplasm, site of sperm deposition and laboratory set-up. The search strategy was limited to articles published in English involving human subjects, with the last search performed on June 2023.This overview provides modern-day knowledge on some technical factors to improve the ICSI efficacy in ART contexts.