EXPRESSION LEVEL OF GENES OF PRO- AND ANTI-INFLAMMATORY CYTOKINES IN THE BLOOD OF COWS WITH OVARIAN HYPOFUNCTION AND THE USE OF DRUG BASED ON GM-CSF

Abstract

The article presents the material for studying the expression level of pro- and anti-inflammatory cytokine genes in the blood of cows with ovarian hypofunction and the use of the drug based on gm-csf (granulocyte-macrophage colony-stimulating factor). It has been established that disorders in the functional activity of the genital gonads in cows occur against the background of an increase in the relative expression level of genes of pro-inflammatory cytokines (IL-1β - by 1.56 and 2.11 times, IL-2 - by 1.41 and 2.29 times, TNFα - by 19.5 and 48.8%), while reducing anti-inflammatory ones (IL-4 - by 27.6 and 37.9%, IL-10 - by 8.3 and 36.1%). An imbalance between the expression level of pro- and anti-inflammatory cytokines in the blood of cows is one of the etiological factors leading to hormonal imbalance and the development of hypofunctional ovarian disorders. The administration of a drug based on gm-csf to cows during insemination once at a dose of 5.0 ml to normalize the functional activity of the ovaries in case of their hypofunctional state with anovulation phenomena contributed to a decrease in the expression level of IL-1β by 27.3%, IL-2 – by 16.7%, TNFα – by 8.2%, with an increase in the expression of IL-4 by 4.8%, IL-10 – by 6.1%, which ensured a clinical efficacy of 86.7%. The use of a drug based on gm-csf in the cows with ovarian hypofunction, manifested by anaphrodisia, twice with an interval of 7 days at a dose of 10.0 ml provided a decrease in the expression level of IL-1β - by 1.9 times, IL-2 – by 38.5%, TNFα – by 14.8%, with an increase in IL-4 - by 11.1%, IL-10 – by 39.1% and a clinical efficacy of 90.0%.