Biometry and pre-germinative treatments of seeds from an Amazon endemic species

Leomara Pessoa Brito, José Dantas Araújo Lacerda, Cintia Araújo Soares, Franklin Luís Carlos Monteiro Júnior, Magno Chagas Souza, Áurea Izabel Aguiar Fonseca e Souza, Clenes Cunha Lima, Marcos Rodrigues
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Abstract

This study aimed to verify the impact of different methods of overcoming dormancy in the germination of Ipomoea cavalcantei seeds, in addition to surveying the biometric data of its seeds and fruits. Ripe fruits were randomly collected from the species’ habitat, which is a unique ecosystem characterized by small vegetation and iron outcrops. For the biometric analyses, the weight, moisture content, length, width, and thickness of 100 fruits and 100 seeds were determined. To analyse the efficiency of overcoming seed dormancy, six different treatments were tested, including mechanical, physical, and chemical methods, and the resultant germinability, average germination time, and germination speed index were evaluated. Further, in order to estimate the imbibition curve, two treatments were selected to measure the water absorption rate of the seeds until the root emission of at least 50 % of the seeds. The results showed that the I. cavalcantei fruits and seeds biometrically vary in length, width, and thickness, which makes it difficult to obtain standard in situ samples. Moreover, mechanical scarification methods were the most efficient treatments to increase the efficiency of obtaining seedlings of this species. This study can contribute to ex situ conservation strategies for this endemic species, which is threatened by increasing mining activities.
一种亚马逊特有种种子的生物计量学和萌发前处理
本研究旨在验证不同的克服休眠方法对卡瓦尔坎蒂伊波莫亚种子萌发的影响,并调查其种子和果实的生物特征数据。在该物种栖息地随机采集成熟果实,该栖息地是一个独特的生态系统,以小植被和铁露头为特征。为进行生物计量分析,测定了100个果实和100个种子的重量、含水量、长度、宽度和厚度。为了分析克服种子休眠的效果,采用机械、物理和化学等6种不同处理方法,对其发芽率、平均发芽时间和发芽速度指数进行了评价。此外,为了估计吸胀曲线,选择两个处理测量种子的吸水率,直到至少50%的种子根部排出。结果表明,卡瓦尔坎泰的果实和种子在长度、宽度和厚度上存在生物特征差异,因此难以获得标准的原位样品。此外,机械划伤法是提高该树种获得幼苗效率的最有效的处理方法。本研究可为该特有物种的迁地保护策略提供参考。
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