THE CHOICE OF INDICATORS FOR THE ECOLOGICAL RATIONING OF PETROLEUM PRODUCTS IN CHESTNUT SOILS

E. I. KOVALEVA, S. YA. TROFIMOV, S. A. SHOBA
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Abstract

The response of the microbial community of soils to dif erent levels of oil contamination in a model experiment was studied according to indicators: basal respiration (BR), basal induced respiration (SIR), carbon of microbial biomass (Cmic), potential denitrif cation (DNF), methanogenesis, catalase activity. The soils are represented by light chestnut (Haplic Kastanozems Sodic) and meadow-chestnut (Gleyic Kastanozems Chromic) drysteppe zone of the Stavropol area, dif ering in the organic matter content, salinity and the content of physical clay. The intensity of BR increased af er 30 days of the crude oil input into the soils and caused the growth of Cmic due to the activation of oil-oxidizing microorganisms in anaerobic zones of soils. DNF varied in light chestnut and meadow-chestnut soils not directly, that was probably due to the level of organic matter content in soils and their salinity. Catalase activity was a sensitive indicator for the petroleum hydrocarbons in light chestnut soil, but salinity was the determining factor in its activity in meadow chestnut soil. Biotesting with the use of worms Eisenia fetidashowed the inability of test organisms to survive in non-contaminated chestnut soils. A significant indicator that did not depend on natural factors in chestnut soils was BR and related indicators. Catalase activity and DNF have limitations on the salinity factor.
栗色土壤中石油产品生态配给指标的选择
通过基础呼吸(BR)、基础诱导呼吸(SIR)、微生物生物量碳(Cmic)、潜在反硝化(DNF)、产甲烷(methanogenesis)、过氧化氢酶活性等指标,研究了土壤微生物群落对不同程度油类污染的响应。土壤以斯塔夫罗pol地区的浅板栗(Haplic Kastanozems Sodic)和草甸板栗(Gleyic Kastanozems Chromic)干草原带为代表,在有机质含量、盐度和物理粘土含量上存在差异。原油进入土壤30 d后,BR的强度增加,由于土壤厌氧区油氧化微生物的活化,导致Cmic的生长。浅栗树和草甸栗树土壤的DNF变化不直接,这可能与土壤有机质含量及其盐度水平有关。过氧化氢酶活性是浅栗子土中石油烃的敏感指标,而盐度是草甸栗子土过氧化氢酶活性的决定因素。用蚯蚓进行的生物试验表明,试验生物不能在未受污染的板栗土壤中生存。栗树土土壤生物量及其相关指标不受自然因子的影响。过氧化氢酶活性和DNF对盐度因素有限制。
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