5 Recombinant human growth hormone

L.M. Fryklund, J.R. Bierich, M.B. Ranke
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引用次数: 35

Abstract

  • 1.

    All batches of Met-hGH examined stimulated statural growth to approximately the same extent. The growth rates measured partly exceeded the results obtained in previous studies with pituitary preparations in the same dosage.

  • 2.

    Under treatment with SI, i.e. the preparation with the highest amount of ECP, high antibody titres with high binding capacity against GH and ECP were found. With SII all antibody determinations showed much lower titres. With Somatonorm (SIII), in the large majority of cases no antibodies were detectable. The titres registered in a few children were low and the binding capacities were negligible.

  • 3.

    The biologically determined somatomedin activity was initially pathologically low. During treatment it rose to supraphysiological levels. Also the radioimmunologically assayed somatomedin and the alkaline phosphatase increased significantly.

  • 4.

    At the start of the first series, two patients showed allergic skin reactions which turned out to be caused by the insufficiently purified preparations. Therapy with extractive preparations was free of such side-effects and fully successful. Both of the patients were atopic. A third child who was also allergic developed after 6–9 months the highest antibody titres seen, combined with a high binding capacity. Also, with this boy, treatment was switched over to pit-hGH, with very good results.

  • 5.

    Two children with pituitary dwarfism already developed in utero high antibody titres against Met-hGH but not against ECP. For this response, neither the Somatonorm nor its impurities can be implicated. Rather, it is the reaction to GH generally, which the organism recognizes as a foreign protein and thus as an antigen. One of the patients stopped growing after nine months. Likewise, pituitary GH did not lead to any further improvement.

5重组人生长激素
1.所有批次的Met-hGH对身高增长的刺激程度大致相同。所测得的生长速率部分超过了先前使用相同剂量的垂体制剂所获得的结果。在SI处理下,即ECP含量最高的制剂中,发现了对GH和ECP具有高结合能力的高抗体滴度。SII的所有抗体检测结果都显示低得多的滴度。与Somatonorm (SIII),在绝大多数情况下,没有检测到抗体。少数儿童登记的滴度较低,结合能力可以忽略不计。生物学测定的生长抑素活性最初在病理上很低。在治疗期间,它上升到超生理水平。放射免疫测定的生长素和碱性磷酸酶也明显升高。在第一个系列开始时,两名患者表现出皮肤过敏反应,结果证明是由于制剂纯化不足引起的。用萃取制剂治疗是没有这样的副作用和完全成功的。这两个病人都是过敏性的。第三个同样过敏的孩子在6-9个月后出现了所见的最高抗体滴度,并具有高结合能力。此外,这个男孩的治疗方法被换成了垂体生长激素,效果非常好。两名患有垂体性侏儒症的儿童在子宫内已经发展出抗Met-hGH的高抗体滴度,但没有抗ECP的抗体滴度。对于这种反应,体细胞及其杂质都不能牵涉其中。相反,它通常是对生长激素的反应,生物体将其识别为外来蛋白质,从而作为抗原。其中一名患者在9个月后停止生长。同样,垂体GH也没有导致任何进一步的改善。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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