Organic matter type and soil texture shape prokaryotic communities during early-stage soil structure formation

IF 2.6 3区农林科学 Q1 AGRONOMY

Journal of Plant Nutrition and Soil Science Pub Date : 2023-09-13 DOI:10.1002/jpln.202300142

Tongyan Yao, Franziska Bucka, Ingrid Kögel-Knabner, Claudia Knief

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引用次数: 0

Abstract

Background

Organic matter (OM) serves as substrate for heterotrophic microbial growth. Soil structure supports microbial life by providing various niches for colonization. Microorganisms in turn contribute to soil structure formation.

Aims

We aim to understand how OM of different origin and soil texture affect prokaryotic community structure and the implications on early-stage soil structure formation.

Methods

An artificial soil incubation experiment was conducted with different types of OM, including bacterial necromass and particulate organic matter (POM) of larger or smaller size (sPOM). The mineral composition was modified to obtain a clay loam, loam, and sandy loam texture. The abundance and composition of a natural microbial inoculum were determined after 30 days of incubation by real-time PCR and 16S rRNA gene sequencing, respectively.

Results

The different OM types had a stronger effect on the prokaryotic community structure and abundance than texture. The necromass treatment supported the most distinct prokaryotic community with the highest abundance and lowest diversity, as well as the most intense formation of water-stable microaggregates in comparison to POM and sPOM treatments. Abundant bacterial taxa in all treatments are known to include extracellular polymeric substance producers, indicating that functional redundancy warrants aggregation by gluing agents. Texture-related effects were most consistent in the POM treatment, where larger prokaryotic populations were observed in the coarser-textured soils with fewer but larger soil pores and lower soil water content.

Conclusions

Differences in prokaryotic community structure and abundance due to OM source indicate that aggregation is dependent on different ecological strategists, a POM-degrading population that promotes aggregation and contributes to necromass formation, and a necromass-degrading consortium in which bacteria play a major role.

Abstract Image

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有机质类型和土壤质地影响早期土壤结构形成过程中的原核生物群落

背景有机物（OM）是异养微生物生长的基质。土壤结构通过提供各种定植壁龛来支持微生物的生命。微生物反过来又促进了土壤结构的形成。目的我们旨在了解不同来源的有机质和土壤质地如何影响原核生物群落结构，以及对早期土壤结构形成的影响。方法采用不同类型的有机质（包括细菌新菌体和较大或较小的颗粒有机质（sPOM））进行人工土壤培养实验。矿物成分经过改良，以获得粘壤土、壤土和砂壤土质地。培养 30 天后，分别通过实时 PCR 和 16S rRNA 基因测序测定天然微生物接种物的丰度和组成。结果不同有机质类型对原核生物群落结构和丰度的影响比质地的影响更大。与 POM 和 sPOM 处理相比，坏死物质处理支持最独特的原核生物群落，丰度最高，多样性最低，形成的水稳定性微集聚物也最多。众所周知，所有处理中的丰富细菌类群都包括胞外聚合物物质生产者，这表明功能冗余需要胶合剂的聚合。与质地相关的影响在 POM 处理中最为一致，在质地较粗的土壤中观察到的原核生物数量较多，这些土壤孔隙较少但较大，土壤含水量较低。结论 OM 来源导致原核生物群落结构和丰度的差异表明，聚集取决于不同的生态策略，一个是促进聚集并有助于坏死物质形成的 POM 降解种群，另一个是细菌在其中发挥主要作用的坏死物质降解联合体。

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来源期刊

Journal of Plant Nutrition and Soil Science 农林科学-农艺学

CiteScore

4.70

自引率

8.00%

发文量

审稿时长

8-16 weeks

期刊介绍： Established in 1922, the Journal of Plant Nutrition and Soil Science (JPNSS) is an international peer-reviewed journal devoted to cover the entire spectrum of plant nutrition and soil science from different scale units, e.g. agroecosystem to natural systems. With its wide scope and focus on soil-plant interactions, JPNSS is one of the leading journals on this topic. Articles in JPNSS include reviews, high-standard original papers, and short communications and represent challenging research of international significance. The Journal of Plant Nutrition and Soil Science is one of the world’s oldest journals. You can trust in a peer-reviewed journal that has been established in the plant and soil science community for almost 100 years. Journal of Plant Nutrition and Soil Science (ISSN 1436-8730) is published in six volumes per year, by the German Societies of Plant Nutrition (DGP) and Soil Science (DBG). Furthermore, the Journal of Plant Nutrition and Soil Science (JPNSS) is a Cooperating Journal of the International Union of Soil Science (IUSS). The journal is produced by Wiley-VCH. Topical Divisions of the Journal of Plant Nutrition and Soil Science that are receiving increasing attention are: JPNSS – Topical Divisions Special timely focus in interdisciplinarity: - sustainability & critical zone science. Soil-Plant Interactions: - rhizosphere science & soil ecology - pollutant cycling & plant-soil protection - land use & climate change. Soil Science: - soil chemistry & soil physics - soil biology & biogeochemistry - soil genesis & mineralogy. Plant Nutrition: - plant nutritional physiology - nutrient dynamics & soil fertility - ecophysiological aspects of plant nutrition.