Comparative efficacy of certain extenders on preservation of liquid boar semen

IF 0.2 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Indian Journal of Animal Sciences Pub Date : 2023-09-13 DOI:10.56093/ijans.v93i9.116312

J KAUR, A K SINGH, S S DHINDSA, P SINGH, S K DAS

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Abstract

This study was carried out to compare the efficacy of certain liquid semen extenders for boar semen preservation. Semen ejaculates (n=30) from three boars (one ejaculate/boar/week) stationed at University pig farm, Ludhiana were collected using dummy sow and extended (1:4) in lactose-sodium salt of EDTA-Egg yolk (LSEEY); glucose sodium salt of EDTA-potassium sodium tartrate-sodium citrate (GEPS); and Modena (MOD). The extended semen was kept at 17°C up to 120 h in a BOD incubator. The semen was assessed for various sperm characteristics and lipid peroxidation at 0, 24, 48, 72, 96 and 120 h of preservation. The results revealed significantly higher percentage of sperm motility, viability, plasma membrane integrity and acrosome integrity in semen extended with MOD thanin GEPS and LSEEY at different preservation times. The mean percentage of in vitro capacitation/acrosome reacted spermatozoa was significantly higher in MOD as compared to GEPS and LSEEY at 96 h and 120 h of storage period. In all the extenders, the mean percentage of most sperm attributes decreased progressively as the storage period increased. Correspondingly, the MDA levels were lower in MOD than in their contemporary extenders at all hours of preservation. In conclusion, Modena exhibited improved sperm parameters and reduced oxidative stress for liquid preservation of boar semen.

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几种膨化剂对公猪液体精液保存效果的比较

本研究比较了几种液体精液填充剂对公猪精液的保存效果。采用假母猪采集卢迪亚那大学猪场3头公猪(1头/周)的射精精液(n=30)，以1:4的比例浸泡在乳糖- edta -蛋黄钠盐(LSEEY)中;edta -酒石酸钠钾-柠檬酸钠葡萄糖钠盐;摩德纳(MOD)。延长后的精液在生化需氧量培养箱中于17°C保存至120 h。在保存0、24、48、72、96和120 h时评估精液的各种精子特征和脂质过氧化。结果表明，在不同保存时间，MOD延长的精液中精子活力、活力、质膜完整性和顶体完整性的百分比均显著高于GEPS和LSEEY。保存96 h和120 h时，MOD组体外获能/顶体反应精子的平均百分比显著高于GEPS和LSEEY组。在所有延长者中，大多数精子属性的平均百分比随着储存期的增加而逐渐降低。相应地，在保存的所有时间内，MOD的MDA水平都低于其同时代的扩展者。综上所述，摩德纳在液体保存公猪精液中表现出改善的精子参数和降低的氧化应激。

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来源期刊

Indian Journal of Animal Sciences 农林科学-奶制品与动物科学

CiteScore

0.60

自引率

25.00%

发文量

220

审稿时长

8 months

期刊介绍： Articles published in The Indian Journal of Animal Sciences encompass a broad range of research topics in animal health and production related to cattle, buffalo, sheep, goat, camel, equines, pig, rabbit, yak, mithun, poultry and fisheries. Studies involving wildlife species and laboratory animal species that address fundamental questions about their biology will also be considered for publication. All manuscripts must present some new development and must be original, timely, significant and scientifically excellent. Papers will be rejected if standards of care of, or procedures performed on animals are not up to those expected of humane veterinary scientists. At a minimum, standards must meet the International Guiding Principles for Biomedical Research involving Animals, as issued by the Council for International Organizations of Medical Sciences. (C.I.O.M.S., c/o WHO, CH 1211 Geneva 27, Switzerland). Articles reporting new animal disease must follow GOI directive as given in detail in Guidelines to Authors.