野生及び飼育ニホンライチョウ未受精卵の免疫グロブリン量の比較

Anne Marit VIK, Sayaka TSUCHIDA, Nami SUZUKI-HASHIDO, Atsushi KOBAYASHI, Yuki AKIBA, Mei HARAFUJI, Kazunari USHIDA
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Abstract

Concentration of immunoglobulin Y (IgY) in egg yolk as well as IgA and IgM in egg white were measured from captive and wild Japanese rock ptarmigan eggs using commercially available chicken Ig ELISA kits, in an effort to assess whether high mortality of chicks in captivity could be attributed to insufficient levels of these antibodies in eggs. Larger but non-significant differences in egg yolk IgY levels were observed between captive and wild specimens using the current methodology, with smaller non-significant differences detected for egg white IgA and IgM. Thus, the lack of maternal passive immunity in the form of egg antibodies might not be a contributing factor to newly hatched chicks’ resistance to opportunistic pathogens under captive conditions.
野生和饲养的原始菌未受精卵中免疫球蛋白含量的比较
利用市售鸡igg ELISA试剂盒,测定了圈养和野生日本石雷鸟蛋的蛋黄免疫球蛋白Y (IgY)浓度以及蛋清中IgA和IgM的浓度,以评估圈养雏鸡的高死亡率是否可归因于鸡蛋中这些抗体水平不足。使用现有方法,在圈养和野生标本之间观察到蛋黄IgY水平存在较大但不显著差异,蛋清IgA和IgM差异较小,但不显著。因此,在圈养条件下,缺乏母体被动免疫(以蛋抗体的形式)可能不是新孵化的雏鸡对机会性病原体产生抗性的一个因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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