Effect of Different Biochemical Parameters and Antioxidant Enzymes Activities on Drought Indices in Chickpea (Cicer arietinum L.)

IF 0.8 4区生物学 Q3 AGRONOMY

Legume Research Pub Date : 2023-10-19 DOI:10.18805/lr-5204

Rajitha Sistu, Sushma Tiwari, M.K. Tripathi, Sangeeta Singh, Neha Gupta, Niraj Tripathi, Ruchi Asati, Rakesh Kumar Yadav

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引用次数: 0

Abstract

Background: Drought is the foremost environmental restraint that effects the growth and yield of chickpea. The mechanism of response to drought differs with genotype and growth stages of individual crop species. The activation of antioxidant enzymes is an alternate defensive system against oxidative stress that ultimately decide development of putative drought tolerant mechanism. Methods: Eighty-three chickpea genotypes were grown under normal and drought stress conditions and leaf samples were used to estimate different biochemical parameters including total sugar, lipid peroxidation (MDA), chlorophyll, proline and protein along with estimation of activities of different antioxidant enzymes viz., catalase (CAT) (EC 1.11.1.6), ascorbate peroxidase (APX) (EC 1.11.1.11), superoxide dismutase (SOD) and peroxidase (POX). Result: Positively significant correlation was found among proline under control with proline underwent stress (r=0.441), MDA under stress with proline under control (r=0.365) and MDA under control (r=0.336) at 1% level of significance. Positively significant correlation was also investigated between SOD under stressed condition with SOD under control (r=0.665), POX (0.449) and APX under stress (0.423), CAT under control (0.471) and CAT under stress condition (0.374) at 1% probability level. Heatmaps along with dendrograms represented expression levels of different antioxidant enzymes activities that showed variations among different genotypes. In conclusion. total sugar, proline and Malondialdehyde, have been increased under drought stressed condition whilst total chlorophyll and protein were decreased. While antioxidant enzymes viz., POX, APX, CAT and SOD levels increased under drought stressed conditions.

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不同生化参数及抗氧化酶活性对鹰嘴豆干旱指标的影响

背景:干旱是影响鹰嘴豆生长和产量的首要环境制约因素。不同作物品种对干旱的响应机制因基因型和生长阶段而异。抗氧化酶的激活是抗氧化胁迫的一种替代防御系统，最终决定了推定的抗旱机制的发展。方法:在正常和干旱胁迫条件下培养83种鹰嘴豆基因型，利用叶片样品测定其总糖、脂质过氧化(MDA)、叶绿素、脯氨酸和蛋白质等生化指标，并测定其过氧化氢酶(CAT) (EC 1.11.1.6)、抗坏血酸过氧化物酶(APX) (EC 1.11.1.11)、超氧化物歧化酶(SOD)和过氧化物酶(POX)的活性。结果:受控制的脯氨酸与受胁迫的脯氨酸呈显著正相关(r=0.441)，受胁迫的MDA与受控制的脯氨酸呈显著正相关(r=0.365)，受控制的MDA呈显著正相关(r=0.336)，在1%的显著水平上。胁迫条件下SOD与对照SOD (r=0.665)、POX (r= 0.449)与APX (r= 0.423)、CAT (r= 0.471)与CAT (r= 0.374)呈1%概率正相关。热图和树状图代表了不同抗氧化酶活性的表达水平，显示了不同基因型之间的差异。在结论。总糖、脯氨酸和丙二醛含量在干旱胁迫下增加，总叶绿素和总蛋白质含量降低。而抗氧化酶(即POX、APX、CAT和SOD)水平在干旱胁迫下升高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Legume Research AGRONOMY-

CiteScore

1.30

自引率

37.50%

发文量

256

审稿时长

6-12 weeks

期刊介绍： Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.