Effects of Seed of Different Mango Cultivars in Triton X-100 Induced Hyperlipdemic Rats

Abstract

Hyperlipidemia is the most prevalent indicator for susceptibility to atherosclerotic heart diseases. The present study investigated the hypolipidemic potential of Mangifera haden, Mangifera pirie and combined extracts of Mangifera haden and Mangifera pirie seeds in Triton X-100 induced hyperlipidemic rats. Before the starting of the experiment, the animals were acclimatized to the laboratory conditions for a period of seven days. At the end of the acclimatization period, each rat was weighed and randomly divided into twelve groups of five animals each, comprising of test animals and control groups. Proximate analysis, toxicity studies, lipid profile parameters were determined in the study. The research study took place in the animal house of Biochemistry Department, University of Nigeria, Nsukka from July 2020 to August 2022. Proximate composition of Mangifera haden and Mangifera pirie seed extracts revealed that the (%) values of protein, lipid, crude fibre, ash, moisture, and carbohydrate are (4.82, 17.18, 4.29, 2.77, 8.39, 62.55) and (3.81, 20.20, 3.0, 1.0, 34.0, 37.99) respectively. Mangifera haden and Mangifera pirie were administered at different doses (200 mg, 300 mg, 500 mg) of the extracts per kilogram body weight of the animals while combined extract of Mangifera haden and Mangifera pirie were administered at 300 mg and 500 mg of the extract per kilogram body weight of the animals in Triton –Induced hyperlipidemic rats. Simvastatin and Atorvastatin were used as reference standards. Simvastatin was found to be an effective drug to Atorvastatin in lowering lipid profiles. The statistical analyses were carried out using one way ANOVA followed by Dunnett’s Post Hoc Multiple Comparisons test. Mangifera haden, Mangifera pirie and combined extracts of both species show significant decrease (P = 0.05) in the levels of serum Total Cholesterol, triacylglycerols, VLDL-C, LDL-C, Atherogenic index (A.I) and significant increase (P = 0.05) of serum HDL-C against Triton-Induced hyperlipidemic rats. The result also suggest that at 200 mg/kg Mangifera pirie and 300 mg/kg Mangifera haden body weight concentrations are an excellent lipid lowering agent. Single administration of either Mangifera haden or Mangifera pirie effectively suppressed the Triton-Induced hyperlipidemia in rats than the combined extracts. The organic extracts (Mangifera haden or Mangifera pirie) exhibited quite hypolipidemic potential when compared with one of the reference drugs, Atorvastatin which indicates that the organic extracts could be explored as an alternative therapeutic agent in the treatment of hyperlipidemia.