Genetic analysis and characterisation of Cmowf, a gene controlling the white petal colour phenotype in pumpkin (Cucurbita moschata D)

IF 1.5 4区农林科学 Q2 AGRONOMY

Plant Breeding Pub Date : 2023-11-05 DOI:10.1111/pbr.13146

Ziyang Min, Yongqi Li, Bo Sun, Jiajia Li, Qi Wu, Zhongyang Liu, Xiaoxia Han, Xinjun Hu

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Abstract

Abstract Flower colour, as an important morphological marker, plays an essential role in improving the identification efficiency of the purity seed in hybrid production. However, the molecular mechanism of white‐flower trait has not been reported in pumpkin ( Cucurbita moschata D.). In this study, we obtained a white‐flower mutant ( wf ) through the ethyl methane sulfonate (EMS) mutagenesis of inbred line N87 (yellow flower). F2 populations were then constructed by crossing wf mutant and N87 plant to fine map the genes controlling white‐flower trait in pumpkin. Phenotypic identification revealed that carotenoid content significantly decreased in the petals of wf mutants compared with N87 plants. Genetic analysis indicated that the white flower mutant trait was controlled by a single recessive gene, Cmowf . Using bulked segregant analysis and KASP phenotyping, Cmowf was mapped to a 762 kb region on chromosome 14 containing three annotated genes. Among them, a nonsynonymous single‐nucleotide polymorphisms mutation was identified only in CmoCh14G005820 gene, which encoded a DUF1997 family protein. Compared with CmoDUF1997 amino acid sequences between the wf mutants and N87 plants, the critical amino acid mutations (early termination of amino acids) occurred in wf mutants, so CmoCh14G005820 was predicted as a potential candidate for controlling the white‐flower trait. RNA‐sequencing analysis revealed that the expression of CmoCh14G005820 and most genes involved in carotenoid biosynthesis was significantly downregulated in wf mutants, whereas the expression of several genes responsible for carotenoid degradation was upregulated in wf mutants. This finding suggested that carotenoid metabolism may participate in the formation of flower colour in pumpkin. Overall, our results provided a theoretical basis for understanding the genetic mechanisms underlying white‐flower formation in pumpkin.

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南瓜白色花瓣颜色表型控制基因Cmowf的遗传分析与特征分析

摘要花色作为一种重要的形态标记，在杂交生产中对提高纯度种子的鉴定效率起着至关重要的作用。然而，南瓜(Cucurbita moschata D.)白花性状的分子机制尚未报道。本研究通过对自交系N87(黄花)的甲烷磺酸乙酯(EMS)诱变获得了一个白花突变体(wf)。通过wf突变体与N87植株杂交构建F2群体，对南瓜白花性状的控制基因进行精细定位。表型鉴定表明，与N87植株相比，wf突变体花瓣中类胡萝卜素含量显著降低。遗传分析表明，该白花突变性状受单隐性基因Cmowf控制。利用大量分离分析和KASP表型分析，将Cmowf定位到14号染色体上一个762 kb的区域，该区域包含三个注释基因。其中，仅在编码DUF1997家族蛋白的CmoCh14G005820基因中发现了非同义单核苷酸多态性突变。与wf突变体和N87植株的CmoDUF1997氨基酸序列比较，发现wf突变体发生了关键氨基酸突变(氨基酸的早期终止)，因此预测CmoCh14G005820是控制白花性状的潜在候选者。RNA测序分析显示，wf突变体中CmoCh14G005820和大多数类胡萝卜素生物合成相关基因的表达显著下调，而wf突变体中一些类胡萝卜素降解相关基因的表达上调。这一发现提示类胡萝卜素代谢可能参与了南瓜花色的形成。总之，我们的研究结果为理解南瓜白花形成的遗传机制提供了理论基础。

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来源期刊

Plant Breeding 农林科学-农艺学

CiteScore

4.40

自引率

5.00%

发文量

审稿时长

3.0 months

期刊介绍： PLANT BREEDING publishes full-length original manuscripts and review articles on all aspects of plant improvement, breeding methodologies, and genetics to include qualitative and quantitative inheritance and genomics of major crop species. PLANT BREEDING provides readers with cutting-edge information on use of molecular techniques and genomics as they relate to improving gain from selection. Since its subject matter embraces all aspects of crop improvement, its content is sought after by both industry and academia. Fields of interest: Genetics of cultivated plants as well as research in practical plant breeding.