New Insights Into The Evolution of Chloroplast Genomes in Ochna Species (Ochnaceae, Malpighiales)

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.1177/11769343231210756

Nguyen Nhat Nam, Nguyen Hoang Danh, Vu Minh Thiet, Hoang Dang Khoa Do

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引用次数: 0

Abstract

Ochnaceae DC. includes more than 600 species that exhibit potential values for environmental ecology, ornamental, pharmaceutical, and timber industries. Although studies on phylogeny and phytochemicals have been intensively conducted, chloroplast genome data of Ochnaceae species have not been fully explored. In this study, the next-generation sequencing method was used to sequence the chloroplast genomes of Ochna integerrima and Ochna serrulata which were 157 329 and 157 835 bp in length, respectively. These chloroplast genomes had a quadripartite structure and contained 78 protein-coding genes, 30 tRNAs, and 4 rRNAs. Comparative analysis revealed 8 hypervariable regions, including trnK_UUU-trnQ_UUG, rpoB-psbM, trnS_GGA-rps4, accD-psaI, rpl33-rps18, rpl14-rpl16, ndhF-trnL_UAG, and rps15-ycf1 among 6 Ochnaceae taxa. Additionally, there were shared and unique repeats among 6 examined chloroplast genomes. The notable changes were the loss of rpl32 in Ochna species and the deletion of rps16 exon 2 in O. integerrima compared to other taxa. This study is the first comprehensive comparative genomic analysis of complete chloroplast genomes of Ochna species and related taxa in Ochnaceae. Consequently, the current study provides initial results for further research on genomic evolution, population genetics, and developing molecular markers in Ochnaceae and related taxa.

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桔黄色植物叶绿体基因组进化的新认识(桔黄色科)

金莲木科。包括600多种，在环境生态、观赏、制药和木材工业中具有潜在价值。尽管对紫堇科植物的系统发育和植物化学物质的研究已经深入开展，但叶绿体基因组数据尚未得到充分挖掘。本研究采用新一代测序方法对长度分别为157 329 bp和157 835 bp的整叶桔梗(Ochna integerrima)和细叶桔梗(Ochna serrulata)叶绿体基因组进行测序。这些叶绿体基因组具有四部结构，包含78个蛋白质编码基因，30个trna和4个rrna。结果显示，6个桔科分类群中存在trnK_UUU-trnQ_UUG、rpoB-psbM、trnS_GGA-rps4、accD-psaI、rpl33-rps18、rpl14-rpl16、ndhF-trnL_UAG、rps15-ycf1等8个高变区。此外，6个叶绿体基因组存在共享重复序列和独特重复序列。与其他类群相比，中国种属rpl32基因缺失，荷叶花种属rps16外显子2缺失。本研究首次对桔黄色属植物及其相关分类群的叶绿体全基因组进行了比较分析。因此，本研究为进一步研究桔科及相关分类群的基因组进化、群体遗传学和开发分子标记提供了初步的结果。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.