Can colistin susceptibility results determined in multidrug resistant Gram-negative bacteria be used for polymyxin B?

IF 0.2 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Postȩpy higieny i medycyny doświadczalnej Pub Date : 2023-01-01 DOI:10.2478/ahem-2023-0017

Rıza Adaleti, Nilgün Kansak, Şeyma Çalık, Neslihan Arıcı, Seniha Şenbayrak, Sebahat Aksaray

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引用次数: 0

Abstract

Abstract Introduction The present study aimed to compare the MIC values of colistin and polymyxin B in fermentative and non-fermentative Gram-negative bacteria and to examine whether the susceptibility results determined with colistin can be used for polymyxin B. Materials and Methods A total of 268 strains of Escherichia coli ( E. coli ), Klebsiella pneumoniae ( K. pneumoniae ), Pseudomonas aeruginosa ( P. aeruginosa ), and Acinetobacter baumannii ( A. baumannii ) isolates were included in the study. The reference broth microdilution and the results were evaluated according to CLSI recommendations. Results Of the 268 strains, 40.7% were found to be colistin resistant and 34% polymyxin B resistant. Essential agreement (EA) and categorical agreement (CA) ratios for all isolates were found to be 81.3% and 85.1%, respectively. Identical MIC values were found for colistin and polymyxin B in 104 isolates (38.8%), and this rate was 31.1% and 48.7% in Enterobacterales and non-fermentative bacteria, respectively. Colistin resistance rates were 25.4% for E. coli , 67.4% for K. pneumoniae , 23.8% for P. aeruginosa , and 31.5% for A. baumannii . Polymyxin B resistance rates were 18.6% for E. coli , 64.1% for K. pneumoniae , 19% for P. aeruginosa , and 16.7% for A. baumannii . Conclusion According to the results of the study, it was concluded that the MIC values of colistin and polymyxin B antibiotics in all bacteria were not compatible with each other. Colistin susceptibility results cannot be used for treatment with polymyxin B, despite CLSI's recommendation.

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多粘菌素耐药革兰氏阴性菌的敏感性结果是否可用于多粘菌素B?

摘要:本研究旨在比较大肠杆菌素和多粘菌素B在发酵和非发酵革兰氏阴性菌中的MIC值，探讨用粘菌素测定的药敏结果是否可用于多粘菌素B。材料与方法共268株大肠杆菌(E. coli)、肺炎克雷伯菌(K. pneumoniae)、铜绿假单胞菌(P. aeruginosa)、鲍曼不动杆菌(鲍曼不动杆菌)分离株纳入研究。对照肉汤微量稀释度和结果按照CLSI推荐值进行评价。结果268株细菌中粘菌素耐药40.7%，多粘菌素B耐药34%。所有分离株的基本一致性(EA)和分类一致性(CA)比率分别为81.3%和85.1%。黏菌素和多粘菌素B的MIC值在104株分离菌中相同(38.8%)，肠杆菌和非发酵菌的MIC值分别为31.1%和48.7%。大肠杆菌耐药率为25.4%，肺炎克雷伯菌为67.4%，铜绿假单胞菌为23.8%，鲍曼不动杆菌为31.5%。大肠杆菌耐药率为18.6%，肺炎克雷伯菌耐药率为64.1%，铜绿假单胞菌耐药率为19%，鲍曼假单胞菌耐药率为16.7%。结论根据研究结果，所有细菌中粘菌素和多粘菌素B抗生素的MIC值不相容。尽管CLSI推荐，粘菌素敏感性结果不能用于多粘菌素B的治疗。

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来源期刊

Postȩpy higieny i medycyny doświadczalnej MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Advances in Hygiene and Experimental Medicine (PHMD) is a scientific journal affiliated with the Institute of Immunology and Experimental Therapy by the Polish Academy of Sciences in Wrocław. The journal publishes articles from the field of experimental medicine and related sciences, with particular emphasis on immunology, oncology, cell biology, microbiology, and genetics. The journal publishes review and original works both in Polish and English. All journal publications are available via the Open Access formula in line with the principles of the Creative Commons licence.